各型FMDV VP1基因表位嵌入型蛋白SLP-MultiVP1对免疫不同小鼠血清IgG亚型的影响

发布时间：2018-03-21 19:11

本文选题：口蹄疫病毒　切入点：抗体亚型　出处：《中国兽医学报》2017年02期 　论文类型：期刊论文

【摘要】：利用通过Swiss-Model在线软件分析所得的乳杆菌S-层蛋白高级结构3D模拟图,应用Swiss-Pbd Viewer软件对嵌入型蛋白SLP-MultiVP1中的3型口蹄疫病毒(FMDV)不同表位位点进行标注,并用嵌入型蛋白SLP-MultiVP1和重组蛋白SLP分别免疫6周龄的BALB/c和昆明小鼠,分离3次免疫后的血清,用Mouse IgG1Ready-SET-Go和Mouse IgG2aReady-SET-Go ELISA试剂盒检测血清中IgG抗体亚型IgG1和IgG2a。通过SPSS统计学分析发现:嵌入型蛋白SLP-MultiVP1组IgG1和IgG2a水平显著高于SLP组(P0.05),其中嵌入型蛋白SLP-MultiVP1免疫组的IgG2a显著高于IgG1(P0.05),但是BALB/c和昆明小鼠之间无显著性差异(P0.05)。该结果提示,嵌入型蛋白SLP-MultiVP1可能倾向于激发Th1类细胞介导的免疫应答,而且无个体差异。
[Abstract]:Using 3D simulation map of Lactobacillus Slayer protein advanced structure obtained by Swiss-Model online software, different epitopes of FMDV3 in embedded protein SLP-MultiVP1 were labeled by Swiss-Pbd Viewer software. The 6-week-old BALB/c and Kunming mice were immunized with embedded protein SLP-MultiVP1 and recombinant protein SLP respectively. Mouse IgG1Ready-SET-Go and Mouse IgG2aReady-SET-Go ELISA kit were used to detect IgG antibody subtype IgG1 and IgG2a in serum. SPSS statistical analysis showed that IgG1 and IgG2a levels in SLP-MultiVP1 group were significantly higher than those in SLP group, and IgG2a in SLP-MultiVP1 immunized group was significantly higher than that in SLP group. The results suggest that there is no significant difference between BALB/c and Kunming mice. The embedded protein SLP-MultiVP1 may be inclined to stimulate the immune response mediated by Th1 cells, and there is no individual difference.
【作者单位】：内蒙古农业大学兽医学院;
【基金】：国家自然科学基金资助项目(31360602)
【分类号】：S852.4

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