全反式视黄酸对3T3-L1前脂肪细胞增殖与分化的影响

发布时间：2018-03-22 00:17

本文选题：全反式视黄酸　切入点：前脂肪细胞　出处：《吉林大学》2015年硕士论文　论文类型：学位论文

【摘要】：本研究旨在探讨全反式视黄酸(all trans retinoic acid,ATRA)对3T3-L1前脂肪细胞增殖与分化的影响。培养3T3-L1前脂肪细胞，分别设置对照组（0mol/L）以及10-9、10-8、10-7、10-6和10-5mol/L浓度的ATRA处理组，分别在第0、2、4、6、7和8d通过MTS比色法检测ATRA对3T3-L1前脂肪细胞增殖的影响；在诱导分化第12d，油红O染色观察3T3-L1前脂肪细胞分化的形态变化，油红O染色提取法分析不同浓度ATRA对3T3-L1前脂肪细胞分化的影响，Real-timePCR技术检测前脂肪细胞分化相关基因mRNA水平的表达。结果如下：（1）在3T3-L1前脂肪细胞生长期间，与对照组（0mol/L）相比，10-9mol/L浓度的ATRA处理组不影响3T3-L1前脂肪细胞的增殖（P0.05）。10-8、10-7、10-6和10-5mol/L浓度的ATRA处理组随着时间的增加，显著抑制了细胞的增殖（P0.01）。其中，10-6和10-5mol/L浓度的ATRA处理组抑制3T3-L1前脂肪细胞增殖的效果最为明显。（2）与对照组（0mol/L）相比，，10-7、10-6和10-5mol/L浓度的ATRA抑制3T3-L1前脂肪细胞的分化(P0.01)，而10-9和10-8mol/L浓度的ATRA对3T3-L1前脂肪细胞分化没有显著影响(P0.05)。与10-7mol/L浓度的ATRA处理组相比，10-5mol/L浓度的ATRA对3T3-L1前脂肪细胞分化的抑制效果更为显著(P0.05)。（3）在诱导分化第12d，10-9、10-8、10-7、10-6和10-5mol/L浓度的ATRA处理组中PPARγ、C/EBPα、LXRα、FAS、SCD-1和Glut-4基因mRNA表达减少，与对照组（0mol/L）比较差异极显著（P0.01）。（4）在诱导分化第12d，10-7、10-6和10-5mol/L浓度的ATRA处理组中SREBP-1c基因mRNA表达上升，与对照组（0mol/L）比较差异极显著（P0.01）。10-8mol/L浓度的ATRA处理组中ACCα基因mRNA表达上升，与对照组（0mol/L）比较差异显著（P0.05）。10-5mol/L浓度的ATRA处理组中β-catenin基因mRNA表达上升，与对照组（0mol/L）比较差异极显著（P0.01）。
[Abstract]:The purpose of this study was to investigate all trans retinoic acid (all trans retinoic acid, ATRA) and the influence on the differentiation of 3T3-L1 cells. The proliferation of cultured 3T3-L1 preadipocytes were set in the control group (0mol/L) and 10-9,10-8,10-7,10-6 and 10-5mol/L concentrations of ATRA groups, respectively in the 0,2,4,6,7 and 8D detected by ATRA MTS the color of 3T3-L1 preadipocyte proliferation; induced differentiation in 12D, to observe the morphological changes of 3T3-L1 preadipocyte differentiation oil red O staining, oil red O staining extraction analysis of different concentrations of ATRA on the differentiation of 3T3-L1 preadipocytes, Real-timePCR technology to detect the expression of preadipocyte differentiation related gene mRNA levels. Results the following:
(1) during 3T3-L1 preadipocyte cell growth, compared with the control group (0mol/L), ATRA treatment group 10-9mol/L concentration did not affect the proliferation of 3T3-L1 preadipocyte (P0.05).10-8,10-7,10-6 and 10-5mol/L concentrations of ATRA groups increased with time, significantly inhibited the cell proliferation (P0.01). Among them, the ATRA treatment group 10-6 and the concentration of 10-5mol/L inhibited the proliferation of 3T3-L1 preadipocytes has the most obvious effect.
(2) and control group (0mol/L), 10-7,10-6 and 10-5mol/L concentrations of ATRA inhibited the differentiation of 3T3-L1 preadipocyte (P0.01), and 10-9 10-8mol/L and concentration of ATRA on the differentiation of 3T3-L1 preadipocytes had no significant effect (P0.05). Compared with the ATRA group of 10-7mol/L concentration, the inhibitory effect of 10-5mol/L concentration of ATRA on the differentiation of 3T3-L1 preadipocytes was more significant (P0.05).
(3) in the ATRA treated group with the concentration of 12D, 10-9,10-8,10-7,10-6 and 10-5mol/L, the expression of PPAR, C/EBP, LXR, FAS, SCD-1 and Glut-4 decreased significantly compared with the control group (SCD-1).
(4) on the differentiation of 12D, SREBP-1c increased the expression of mRNA gene in ATRA treated 10-7,10-6 and 10-5mol/L concentration, and the control group (0mol/L) significantly increased ACC (P0.01) mRNA gene expression in ATRA treated.10-8mol/L concentration, and the control group (0mol/L) and there was significant difference (P0.05) increased beta -catenin gene expression of mRNA ATRA.10-5mol/L concentration in treatment group, and control group (0mol/L) significantly (P0.01).

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S816

【参考文献】