H9N2亚型禽流感病毒河南分离株的全基因克隆及序列分析

发布时间：2018-03-22 01:08

本文选题：禽流感病毒　切入点：HN　出处：《黑龙江畜牧兽医》2017年11期 　论文类型：期刊论文

【摘要】：为了研究H9N2亚型禽流感病毒的基因组变异情况,试验以一株2015年在河南省分离的H9N2亚型Ck/HN/12/15为研究对象,用RT-PCR方法扩增病毒基因组的8个基因片段并进行克隆和测序,将测序结果与Gen Bank公布的H9N2参考毒株序列进行比对分析,绘制各基因片段的进化树。结果表明:分离株的PB1、PA、NP、NS基因与1998年中国上海鸡源毒株Ck/SH/F/98(H9N2)归于同一进化分支,核苷酸序列同源性分别为93.57%、90.48%、87.66%和86.64%。分离株的HA、NA基因与1997年中国香港鸭源毒株Dk/HK/Y280/97(H9N2)属同一个进化分支,核苷酸序列同源性分别为87.62%和85.32%。该毒株裂解位点处的序列是PSRSSR,符合低致病性禽流感病毒的分子特点。该株HA基因的226位(对应H3亚型位置)氨基酸为Leu,更易与哺乳动物唾液酸受体结合。说明应该做好对此类H9N2亚型禽流感病毒免疫防控工作,避免在人和禽类之间传播。
[Abstract]:In order to study the genomic variation of H9N2 subtype avian influenza virus, a H9N2 subtype Ck/HN/12/15 isolated in Henan province in 2015 was used to amplify, clone and sequence eight gene fragments of the virus genome by RT-PCR. The results of sequencing were compared with the sequence of H9N2 reference strain published by Gen Bank, and the phylogenetic tree of each gene fragment was plotted. The results showed that the PB1PPANPNS gene of the isolated strain belonged to the same evolutionary branch as the 1998 Shanghai chicken strain CK / SHR / F / 98 H9N2. The homology of nucleotide sequence was 90.48% and 86.64%, respectively. The HANNA gene of the isolated strain belonged to the same evolutionary branch as DkP / HKR / Y280/ 97H9N2 strain from Hong Kong, China, in 1997. The nucleotide sequence homology was 87.62% and 85.322.The nucleotide sequence at the cleavage site of the strain was PSRSSRS, which was in line with the molecular characteristics of the low pathogenic avian influenza virus. The amino acid of the HA gene of the strain was Leu (corresponding to H3 subtype). The sialic acid receptor binding in milk animals suggests that we should do a good job in the prevention and control of this H9N2 subtype avian influenza virus. Avoid spreading between people and birds.
【作者单位】：华南农业大学动物科学学院;
【基金】：2016年“攀登计划”广东大学生科技创新培育专项基金项目(pdjh2016a0073)
【分类号】：S852.65

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