氨基丁三醇前列腺素F2α在奶牛体内的药代动力学研究

发布时间：2018-03-22 05:09

本文选题：奶牛　切入点：氨基丁三醇PGFα注射液　出处：《中国兽医学报》2017年05期 　论文类型：期刊论文

【摘要】：将18头健康成年中国荷斯坦奶牛随机分成高、中、低3组,每组每头分别肌肉注射氨基丁三醇前列腺素F2α注射液10(50mg),5(25mg),2.5mL(12.5mg),采用酶联免疫吸附法(ELISA)测定血药浓度,用残数法逐头奶牛拟合药动学曲线方程并计算药动学参数,研究氨基丁三醇前列腺素F2α在奶牛体内的药代动力学。结果表明,药时数据符合有吸收一室模型,高,中,低组的主要药动学参数:吸收速率常数Ka分别为(18.22±2.17),(15.30±0.64),(20.09±5.03)h-1;吸收半衰期t1/2Ka分别为(0.04±0.004),(0.05±0.002),(0.04±0.01)h;分布和消除速率常数Ke分别为(1.32±0.14),(1.27±0.12),(1.40±0.16)h-1;消除半衰期t1/2Ke分别为(0.53±0.06),(0.55±0.05),(0.50±0.06)h;达峰时间tmax均为0.17h;达峰质量浓度Cmax分别为(6.34±0.59),(5.94±0.27),(4.81±0.16)μg/L;药时曲线下面积AUC0~t分别为(14.40±2.19),(10.14±1.07),(13.15±4.34)μg/(L·h)。奶牛单剂量肌肉注射氨基丁三醇前列腺素F2α注射液后,机体对药物吸收迅速,消除快,浓度约10min达到峰值,100min后达到注射前的水平。根据药时曲线峰浓度,AUC值及相关的临床药效学试验结果,建议临床使用剂量为5mL(25mg)。
[Abstract]:Eighteen healthy adult Chinese Holstein cows were randomly divided into 3 groups: high, middle and low. Each group was given intramuscular injection of ABA prostaglandin F _ 2 伪 injection 10 ~ 50 mg / L ~ 2.5 mL / L ~ 12.5 mg 路g ~ (-1) 路L ~ (-1) 路L ~ (2.5) mg 路L ~ (-1) ~ (-1) 路L ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)). The pharmacokinetics of aminobutanol prostaglandin F 2 伪 in dairy cows was studied by fitting the pharmacokinetic curve equation and calculating the pharmacokinetic parameters with the residue method. The results showed that the pharmacokinetics of ABA prostaglandin F 2 伪 was consistent with the absorption one-compartment model. The main pharmacokinetic parameters of the low group were as follows: the absorption rate constant Ka was 18.22 卤2.17, the absorption half-life t1/2Ka was 0.04 卤0.004, the absorption rate constant K _ e was 1.32 卤0.14 and 1.27 卤0.12, respectively; the half-life t1/2Ke was 0.53 卤0.06, 0.55 卤0.06, respectively; the peak time was 0.17h. The Cmax was 6.34 卤0.59 渭 g / L and the area under the curve was 10.14 卤1.07 卤1.07 渭 g / L, respectively. After single dose injection of prostaglandin F _ 2 伪, aminobutanol prostaglandin F _ 2 伪 was injected intramuscularly in dairy cows. The concentration of 10min reached the level before injection. According to the peak concentration of AUC and the results of clinical pharmacodynamics test, it was suggested that the clinical dosage should be 5 mL / L ~ 25 mg / g ~ (-1) ~ (-1) 路min ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)).
【作者单位】：西北农林科技大学动物医学学院;
【基金】：西北农林科技大学国家级“大学生创新创业训练计划”项目(201510712023)
【分类号】：S859.793

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