猪源大肠杆菌O157∶H7毒力差异株的转录组测序分析

发布时间：2018-03-24 05:45

本文选题：大肠杆菌O∶H　切入点：转录组　出处：《中国畜牧兽医》2017年06期

【摘要】：为了解大肠杆菌O157∶H7毒力差异株转录组差异,丰富O157∶H7转录组数据信息,本研究采用Illumina HiSeqTM2000平台对两株大肠杆菌O157∶H7毒力差异株进行高通量测序,测序数据采用测序评估、基因功能注释等生物信息学方法进行分析。结果发现,经过测序,两个菌株分别获得3 113 118和2 944 912条reads,比对到参考基因组上的reads分别占总reads的83.76%和78.97%。以中等毒力株为参考,在高毒力株中共获得941个差异表达基因,其中上调基因637个,下调基因304个。GO功能注释分析表明,差异表达基因主要与催化活性功能、黏附、转运活性、受体活性、酶调节活性、定位、生化调节、运动等诸多生理生化过程相关;KEGG富集分析发现共有425个基因注释到160个代谢通路中,其中新陈代谢、核糖体、鞭毛合成、嘧啶代谢、糖类代谢、细菌趋化等通路显著富集。此次通过大肠杆菌O157∶H7毒力差异株转录组研究对差异表达基因涉及的信号调控及可能的功能基因进行了探索,丰富了转录组信息,为进一步开展大肠杆菌O157∶H7毒力相关基因的研究及分子调控机制奠定了基础。
[Abstract]:In order to understand the virulence of Escherichia coli O157: H7 strains transcriptome differences, rich O157: H7 transcriptome data, this study uses the Illumina HiSeqTM2000 platform of two strains of Escherichia coli O157: H7 virulence strains were high-throughput sequencing, sequencing data by sequencing evaluation, gene function annotation methods, bioinformatics analysis results. Found that, after sequencing, two strains were respectively 3113118 and 2944912 reads, compared to the reference genome reads accounted for 83.76% of total reads and 78.97%. in the middle of a virulent strain as a reference, 941 differentially expressed genes in the high virulent strains, including 637 up-regulated genes and 304 down regulated genes.GO functional annotation analysis show that the differentially expressed genes mainly related to catalytic activity, transporter activity, adhesion, receptor activity, enzyme regulator activity, localization, biochemical regulation, movement and many other physiological and biochemical processes; KEGG enrichment analysis showed that there were 425 genes annotated to 160 metabolic pathways, including flagellar synthesis, ribosome, The new supersedes the old., pyrimidine metabolism, carbohydrate metabolism, bacterial chemotaxis pathway. The significant enrichment of Escherichia coli O157: H7 virulence difference of plant transcriptome studies on differential expression genes involved in signal regulation and gene function may be explored to enrich the transcriptome information for the further development of Escherichia coli O157: laid the foundation for research and molecular regulation mechanism of H7 gene related to virulence.

【作者单位】：广西兽医研究所;广西兽医生物技术重点实验室;广西大学动物科学技术学院;
【基金】：广西科技厅攻关项目(桂科攻0719004-3C) 广西畜禽疫苗新技术重点实验室专项(14-045-31-B-4)
【分类号】：S852.61

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