褪黑素调节雄性大鼠脂肪代谢的研究

发布时间：2018-03-24 13:33

本文选题：褪黑素　切入点：脂肪沉积　出处：《畜牧兽医学报》2017年09期

【摘要】：旨在研究褪黑素(Melatonin,MT)对雄性大鼠脂肪代谢的影响及其对不同脂肪代谢酶/因子的作用,初步揭示其调节脂肪代谢的机理。选择20只日龄、体重接近的SD雄性大鼠随机平均分为2组,分别为对照组(ND)和褪黑素处理组(ND+MT)。ND+MT组除饲喂正常的饲粮外,每天灌服10mg·kg-1 BW的MT溶液。试验60d后采血,分析血液生化、激素及脂代谢相关指标;试验结束后处死大鼠,摘取肝组织,分离腹部各部位脂肪组织并称重,采集肝组织样品用于基因芯片检测和相关分子分析。结果表明:1)ND+MT组大鼠肝重、肝指数、大网膜重、大网膜指数、附睾脂肪重显著降低(P0.05)。血清低密度脂蛋白显著降低(P0.05),高密度脂蛋白、总胆固醇含量下降,极低密度脂蛋白含量升高;肝组织中长链脂肪酸含量显著降低(P0.05)。2)基因芯片和荧光定量PCR检测结果表明,肝组织中脂肪分解相关因子CPT1的mRNA表达显著上调(P0.05),脂肪分解相关酶ALDH3a2的mRNA表达升高(0.05P0.1),脂肪合成相关酶ACSL3、FAS的mRNA表达显著下调(P0.05)。结果提示,MT可显著影响脂肪的代谢,减少内脏脂肪的含量,其机制与相关激素及肝脂肪代谢相关酶(因子)对MT的不同反应有关。
[Abstract]:The aim of this study was to study the effects of melatonin on fat metabolism in male rats and its effects on different fat metabolism enzymes / factors, and to reveal its mechanism of regulating fat metabolism. Sprague-Dawley male rats with close body weight were randomly divided into two groups: control group (ND) and melatonin treated group (ND MT).ND MT group). In addition to feeding normal diet, 10mg kg-1 BW MT solution was administered daily. Blood samples were collected for 60 days and blood biochemistry was analyzed. At the end of the experiment, the rats were killed, the liver tissues were removed, the adipose tissues were isolated and weighed, and the liver tissue samples were collected for gene chip detection and molecular analysis. The results showed that the liver weight of the rats in the group of 1: 1 ND MT was higher than that in the control group. Liver index, omentum weight, omentum index and epididymal fat weight decreased significantly (P 0.05), serum low density lipoprotein (LDL) significantly decreased P 0.05N, high density lipoprotein (HDL), total cholesterol content decreased, and very low density lipoprotein (VLDL) content increased. The content of long chain fatty acids in liver tissue was significantly decreased by P0.05. 2) gene chip and fluorescence quantitative PCR. The mRNA expression of adipose catabolism related factor (CPT1) was significantly up-regulated in liver tissue, the mRNA expression of adipocate-related enzyme (ALDH3a2) was increased by 0.05P0.1, and the mRNA expression of fatty synthase ACSL3FAS was significantly down-regulated. The results suggested that MMT could significantly affect the metabolism of fat. The mechanism of decreasing visceral fat content is related to the different reactions of related hormones and hepatic fat metabolism related enzymes (factors) to MT.
【作者单位】：河南农业大学牧医工程学院农业部动物生化与营养重点试验室;河南农业大学现代实验技术与管理中心;
【基金】：国家现代奶牛产业技术体系建设专项(CARS-37) 河南省现代农业产业技术体系专项基金(2013-14)
【分类号】：S852.2

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