大围山微型鸡和艾维茵肉鸡骨骼肌卫星细胞增殖分化及Myf5基因表达差异研究

发布时间：2018-03-24 22:24

本文选题：大围山微型鸡　切入点：艾维茵肉鸡　出处：《中国家禽》2017年11期

【摘要】：为研究不同鸡种骨骼肌卫星细胞生肌决定因子5(Myf5)基因表达差异,试验通过提取大围山微型鸡和艾维茵肉鸡骨骼肌卫星细胞并进行两个鸡种骨骼肌卫星细胞增殖分化差异及Myf5基因表达差异研究。结果显示:两个鸡种骨骼肌卫星细胞生长曲线呈"S"形,大围山微型鸡骨骼肌卫星细胞生长速度较低;在分化过程中,艾维茵肉鸡骨骼肌卫星细胞分化能力高于大围山微型鸡,且艾维茵肉鸡骨骼肌卫星细胞的Myf5 mRNA表达量显著高于大围山微型鸡(P0.05)。本试验表明:Myf5基因是影响家禽肌肉生长发育的重要候选基因,且细胞体外培养对家禽分子育种研究具有一定科学指导意义。
[Abstract]:In order to study the difference of myogenic determinant 5 (Myf5) gene expression in different chicken skeletal muscle satellite cells, In this experiment, the skeletal muscle satellite cells were extracted from Daweshan miniature chicken and Avian broiler, and the differences of proliferation, differentiation and Myf5 gene expression between the two chicken skeletal muscle satellite cells were studied. The results showed that: two chicken skeletal muscle satellites. The cell growth curve was "S" shape. The growth rate of skeletal muscle satellite cells in Daweshan miniature chicken was lower, and the differentiation ability of Avian broiler skeletal muscle satellite cells was higher than that of Daweishan miniature chicken. The expression of Myf5 mRNA in the skeletal muscle satellite cells of Avian broilers was significantly higher than that of P0.05 in Daweshan miniature chicken. This study showed that the 1: Myf5 gene is an important candidate gene affecting the growth and development of poultry muscle. And cell culture in vitro has certain scientific guiding significance for poultry molecular breeding.
【作者单位】：云南农业大学动物科学技术学院;云南农业大学食品科学技术学院;
【基金】：“十三五”农村领域国家科技计划项目(2015BAD03B03-4) 国家自然科学基金项目(31572359,31560618) 云南省科技计划重点项目(2016FA046)
【分类号】：S831

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