新疆南疆绵羊的泰勒虫感染情况及种类鉴定

发布时间：2018-03-25 05:13

本文选题：泰勒虫　切入点：流行情况　出处：《塔里木大学》2017年硕士论文

【摘要】：羊泰勒虫病是经媒介蜱传播的一种血液原虫病,该病主要可引起感染动物高热、体表淋巴结肿大、贫血和消瘦等症状,严重者可致死亡。该病呈地方流行性,尤其对我国西北地区养羊业危害较大。国内外有关羊血液内泰勒虫病的流行情况和检测方法研究较多,但对于新疆南疆地区绵羊的泰勒虫病感染情况和病原种类的报道尚较少。本研究旨在应用显微镜检查和PCR技术查明该区域绵羊血液中泰勒虫的感染情况,并鉴定其病原种类,以期为养羊业中羊泰勒虫病的诊断和防治提供科学依据。1.为了解喀什地区绵羊泰勒虫病的流行情况,于2015年11月至2016年1月,采用血涂片镜检法对喀什11个(区、县)258只当地成年绵羊的血液样品进行检查。结果发现泰勒虫阳性样品151份,泰勒虫总感染率为59.0%。不同采样点绵羊的泰勒虫感染率存在差异,其中莎车绵羊的泰勒虫感染率最高达75%,而喀什市绵羊的泰勒虫感染率较低,为8.3%。调查结果表明,喀什地区绵羊的泰勒虫病普遍流行,应加强对该病的综合防治。2.为进一步掌握新疆南疆绵羊的泰勒虫感染情况及其种类。基于泰勒虫通用引物,采用PCR法对该区域637份绵羊血液DNA样品进行检测,发现泰勒虫阳性样品400份,总感染率为62.8%。不同采样点绵羊的泰勒虫感染率存在差异,其中,莎车、英吉沙和泽普绵羊的泰勒虫感染率均高达100%,沙雅绵羊的泰勒虫感染率较低,为4.0%。分别用绵羊泰勒虫、尤氏泰勒虫和吕氏泰勒虫特异性引物对以上400份泰勒虫阳性样品进行扩增,结果显示,绵羊泰勒虫特异性引物扩增出399个阳性,而尤氏泰勒虫和吕氏泰勒虫均未扩增出。1份样品(样品编号为1443)在通用引物位点鉴定为阳性,但在3种特异性引物均未扩增出条带。3.随机选取PCR检测为绵羊泰勒虫的阳性样品中的56个样本和1443号样本进行测序,成功获得55个绵羊泰勒虫序列和1443号样本序列。在NCBI中进行Blast比对,应用Clustal X 2.11软件分别进行比对及序列拼接。发现所有绵羊泰勒虫序列均一致,与来自的伊朗的绵羊源绵羊泰勒虫(GenBank序列登录号为KX273858)、土耳其的绵羊源绵羊泰勒虫(KU342701)、中国的绵羊源绵羊泰勒虫(FJ603460)序列同源性为100%,鉴定为绵羊泰勒虫。1443号样本的序列与来自的伊朗的牛源环形泰勒虫(KX273857)、印度的牛源环形泰勒虫(KT736498)、中国的梅花鹿源环形泰勒虫(KT959231)序列同源性为100%,鉴定为环形泰勒虫。本研究应用显微镜检查和PCR技术对新疆南疆绵羊血液样品进行了泰勒虫检测,发现该区域绵羊的泰勒虫感染较为普遍,且发现了环形泰勒虫。本研究为绵羊的泰勒虫病的传播、诊断和防治提供了参考依据,并为绵羊泰勒虫病的免疫学研究提供有益资料。
[Abstract]:Taylor's disease of sheep is a blood protozoa disease transmitted by vector ticks. It can cause high fever, enlarged lymph nodes, anemia and wasting in infected animals. In particular, it is harmful to sheep farming in northwest China. There are many studies on the prevalence and detection methods of Taylor's disease in sheep blood at home and abroad. However, there are few reports of Taylor's disease infection and pathogenic species in sheep in southern Xinjiang. The purpose of this study was to use microscope and PCR technique to find out the infection of Taylor's parasite in sheep blood and to identify its pathogenic species. In order to provide scientific basis for diagnosis and control of sheep Taylor's disease in sheep industry, in order to understand the epidemic situation of Taylor's disease in sheep in Kashi area, from November 2015 to January 2016, blood smear microscopy was used to detect 11 areas of Kashi. The blood samples of 258 local adult sheep were examined. The results showed that 151 positive samples of Taylor worm were positive and the total infection rate of Taylor worm was 59.0. There were differences in the infection rate of Taylor parasite in sheep from different sampling points. The highest rate of Taylor infection in sheep was 75 and the infection rate of Taylor worm in sheep in Kashi was 8.3.The results showed that Taylor's disease was prevalent in sheep in Kashi area. In order to further understand the situation and species of Taylor's infection in sheep in southern Xinjiang, 637 sheep blood DNA samples were detected by PCR method based on universal primer of Taylor worm. It was found that there were 400 positive samples of Taylor worm, and the total infection rate was 62.8. There were differences in the infection rate of Taylor parasite in sheep with different sampling points, among which, the infection rate of Taylor parasite was as high as 100% in Sharp, Ingisha and Zep sheep, and the infection rate of Taylor worm in Shaya sheep was lower than that in Shaya sheep. Four hundred positive samples of Taylor's worm were amplified by using specific primers of Taylor's worm, Taylor's worm of sheep and Taylor's worm of lui respectively. The results showed that 399 positive samples were amplified by specific primers of Taylor's worm of sheep. However, none of the 1. 1 samples (sample number 1443) was identified as positive by universal primer locus. However, no bands were amplified from the three specific primers. Random selection of 56 samples and 1443 samples from positive samples of Taylor's worm detected by PCR was carried out for sequencing. 55 sheep Taylor worm sequences and 1443 sample sequences were successfully obtained. Blast alignment was carried out in NCBI, and Clustal X 2.11 software was used to carry out alignment and sequence splicing respectively. It was found that all the sheep Taylor worm sequences were identical. The sequence homology with the GenBank sequence of the sheep from Iran was KX273858, that of Turkey from Turkey was KU342701, and that of China from China was 100%, and that it was identified as the sample of Taylor worm of sheep .1443. The homology of KX273857 with KX273857 from Iran, KT736498 from India and KT959231 from Chinese sika deer was 100. The blood samples of sheep in southern Xinjiang were tested for Taylor worm. It was found that the infection of Taylor's worm in sheep in this area was more common and the circular Taylor worm was found. This study provided a reference for the transmission diagnosis and control of Taylor's disease in sheep and provided useful information for the immunological study of Taylor's disease in sheep.
【学位授予单位】：塔里木大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.26

【共引文献】