丁酸钠对高精料日粮模式下奶山羊乳腺内组胺介导的炎症反应的影响

发布时间：2018-03-25 21:34

本文选题：高精料日粮　切入点：丁酸钠　出处：《畜牧与兽医》2017年09期

【摘要】：本试验旨在研究饲喂高精料日粮以及日粮中添加丁酸钠对奶山羊乳腺内组胺介导的炎症反应的影响。选用泌乳中期的18头萨能奶山羊,随机分成3组,每组各6头。对照组(LC)饲喂粗精比为6∶4的低精料日粮,即日粮包括60%粗料和40%精料;试验一组(HC)饲喂粗精比为4∶6的高精料日粮,即日粮包括40%粗料和60%精料;试验二组(BHC)饲喂粗精比为4∶6的高精料日粮,并在日粮中添加20 g丁酸钠。试验期为20周。试验开始前两周,所有试验动物手术安装瘤胃瘘管。试验第20周采集瘤胃液、外周血液和乳腺组织样品。结果显示:与LC组相比,HC组瘤胃液、外周血液、乳腺组织中的组胺含量显著升高,外周血液中的炎性因子IL-1β、IL-6和TNF-α显著升高,乳腺组织中的组胺受体基因表达显著升高,炎性因子在基因和蛋白表达水平上均显著升高。与HC组相比,BHC组瘤胃液、外周血液、乳腺组织中的组胺含量显著下降,外周血液中的炎性因子均显著降低,乳腺组织中的组胺受体基因表达显著降低,炎性因子的基因和蛋白表达显著下降。结果表明:泌乳奶山羊长期饲喂高精料日粮后,瘤胃产生的组胺经消化道吸收并转运到乳腺组织中,刺激炎症的产生;而通过添喂丁酸钠,组胺和炎性因子的产生显著降低,乳腺组织炎症反应减轻。
[Abstract]:The aim of this study was to study the effects of high concentrate diet and sodium butyrate supplementation on histamine-mediated inflammation in mammary glands of dairy goats. Eighteen Sanen dairy goats were randomly divided into 3 groups. The control group (LCL) was fed a low concentrate diet with a crude concentrate ratio of 6:4, that is, the diet consisted of 60% crude feed and 40% concentrate, and the test group 1 fed a high concentrate diet with a crude concentrate ratio of 4:6, that is, the diet consisted of 40% crude feed and 60% concentrate. Group 2 was fed a high concentrate diet with a crude concentrate ratio of 4:6, and 20 g sodium butyrate was added to the diet for 20 weeks. Two weeks before the beginning of the trial, all experimental animals were operated on to install rumen fistula. Rumen fluid was collected at the 20th week of the trial. The results showed that histamine content in rumen fluid, peripheral blood fluid and mammary gland tissue in HC group was significantly higher than that in LC group, while the inflammatory factor IL-1 尾 -IL-6 and TNF- 伪 in peripheral blood were significantly increased. Histamine receptor gene expression was significantly increased in breast tissues, and inflammatory factors were significantly increased in gene and protein levels. Histamine content in rumen fluid, peripheral blood fluid and breast tissues in BHC group was significantly lower than that in HC group. The expression of histamine receptor gene and inflammatory factor protein were significantly decreased in peripheral blood, histamine receptor gene and protein in breast tissue. The results showed that the lactating goats were fed with high concentrate diet for a long time. Histamine produced by rumen was absorbed by digestive tract and transported to mammary gland to stimulate inflammation, while histamine and inflammatory factors were significantly reduced by adding sodium butyrate.
【作者单位】：南京农业大学动物医学院;
【基金】：国家自然科学基金资助项目(31672618)
【分类号】：S827.5

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