真胃灌注缺失单一必需氨基酸的氨基酸混合物对泌乳山羊乳腺氨基酸代谢的影响研究

发布时间：2018-03-28 00:03

本文选题：奶羊　切入点：AA　出处：《山东农业大学》2015年硕士论文

【摘要】：依据反刍动物泌乳优先保障的原理,当一种必需氨基酸处于泌乳第一限制性位置时,其泌乳转化效率最高。本课题通过真胃灌注缺失一种必需氨基酸的氨基酸混合溶液,依次使各必需氨基酸处于反刍动物泌乳过程的第一限制性状态,研究其在泌乳性能及乳腺氨基酸代谢方面上效应,从而获得反刍动物泌乳代谢中理想氨基酸模式的研究方法。选用4只体况良好经产莎能奶山羊,产乳量在1.5±0.25kg,体重在50±5kg,安装真胃瘘管插管。采用两组4×4拉丁方试验设计。试验羊饲喂基础日粮满足维持的能量和蛋白需要,真胃连续24h灌注葡萄糖和氨基酸混合溶液以满足泌乳的代谢能和代谢蛋白需要。灌注的混合氨基酸处理分别为TAA(含EAA和NEAA)、缺失Arg、缺失Thr或缺失His以及缺失Lys、缺失Met或缺失Leu的氨基酸混合物。观察缺失氨基酸对泌乳代谢和乳腺氨基酸代谢方面的影响。研究结果表明:(1)真胃灌注混合氨基酸缺失Arg、缺失Thr以及缺失Lys、缺失Met或缺失Leu,血流量分别上升22.2%、20.6%、8.7%、11.2%和16.2%。(2)真胃灌注混合氨基酸缺失Arg、缺失Thr、缺失Lys、缺失Met或缺失Leu处理,缺失氨基酸的动脉浓度均明显下降,对应静脉浓度也显著下降,但是除缺失Leu处理对应氨基酸的动静脉浓度差显著下降,乳腺吸收量有下降趋势外,缺失氨基酸对动静脉浓度差和乳腺吸收量影响不显著,但乳腺清除率分别上升25.9%、199%、104.9%、151%和44%。对对应氨基酸U:O值影响不显著。(3)真胃灌注混合氨基酸除缺失Arg、缺失Thr或缺失His以及缺失Lys、缺失Met或缺失Leu,对乳腺泌乳性能影响不显著,仅缺失Arg和Leu,使乳脂含量和产量有显著上升。缺乏Leu和Thr,乳蛋白产量有下降的趋势(4)真胃灌注混合氨基酸缺失Arg、缺失Thr、缺失Lys、缺失Met或缺失Leu处理,显著提高了对应氨基酸的乳腺泌乳效率。本试验研究证明:乳蛋白合成和分泌对氨基酸灌注缺乏造成的动脉氨基酸浓度不平衡并不敏感,乳腺乳蛋白分泌在对氨基酸的吸收和转运以及乳蛋白合成方面均具有很强的自身调节能力。
[Abstract]:According to the principle of ruminant's lactation priority, when an essential amino acid is in the first restricted position of lactation, its lactation conversion efficiency is the highest. In turn, the essential amino acids were placed in the first restrictive state in the lactation process of ruminants, and the effects of these amino acids on lactating performance and amino acid metabolism in mammary glands were studied. Thus, an ideal amino acid pattern in lactating metabolism of ruminants was obtained. The milk yield was 1.5 卤0.25 kg and the body weight was 50 卤5 kg. Two groups of 4 脳 4 Latin square test designs were used. The mixture of glucose and amino acid solution was perfused for 24 hours to meet the metabolic energy and metabolic protein needs of lactation. The mixed amino acids were TAA (containing EAA and NEA A), deletion of Arg, deletion of Thr or His, deletion of lys, deletion of His, respectively. Met or a mixture of amino acids lacking Leu. Effects of absent amino acids on lactating metabolism and amino acid metabolism in breast gland were observed. The results showed that the mixture of amino acids, Thr, Met, Met, or malondialdehyde (Met) were absent in eurogastric perfusion. The loss of Leu, the increase of blood flow (22.2g%) and the increase of blood flow (8.7g% and 16.2g%) were found in the true stomach. The mixture of amino acids was deficient in Arg, Thr, Lys, Met or Leu. The arterial concentration and the corresponding venous concentration of the missing amino acids decreased significantly, but in addition to the difference in the arteriovenous concentration of the corresponding amino acids in the absence of Leu, the absorption of mammary gland tended to decrease. The absence of amino acids had no significant effect on the difference of arteriovenous concentration and mammary absorption. However, the breast clearance rate increased by 25.9% and 44%, respectively. There was no significant effect on the corresponding amino acid U: O.) the mixed amino acids were not significantly affected by the addition of Arg, deletion of Thr or His, deletion of lys, deletion of Met or deletion of Leu, which had no significant effect on the lactating performance of mammary gland. The absence of Arg and Leu only increased the milk fat content and yield significantly, but in the absence of Leu and Thr, the milk protein yield tended to decrease. 4) the mixture of amino acid, Thr, Lys, Met or Leu were deleted in the true stomach. The results showed that the synthesis and secretion of milk protein were not sensitive to the imbalance of amino acid concentration caused by amino acid perfusion. Mammary milk protein secretion has strong self regulation ability in amino acid absorption and transport as well as milk protein synthesis.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S827

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