牛副流感3型病毒Nano-PCR、LAMP方法的建立及初步应用

发布时间：2018-03-29 16:49

本文选题：牛副流感型病毒(BPIV)　切入点：Nano-PCR　出处：《中国畜牧兽医》2017年10期

【摘要】：试验旨在建立牛副流感3型病毒(bovine parainfluenza type-3 virus,BPIV3)纳米PCR(Nano-PCR)与环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)新型快速分子检测技术,对其进行特异性和敏感性对比试验,并对10份临床样品进行了检测。特异性与敏感性试验结果显示,建立的Nano-PCR和LAMP方法只对BPIV3特异,而对牛呼吸道合胞体病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒无交叉反应;建立的Nano-PCR和LAMP方法具有相同的敏感性,均是普通PCR的10倍,最低核酸检出量均为4.16×102拷贝/μL。临床检测结果显示,建立的两种方法阳性符合率为100%,且阳性检出率均高于普通PCR。因此,本试验建立的Nano-PCR和LAMP方法为BPIV3的临床诊断提供了更快速、敏感、可靠的工具。
[Abstract]:The aim of this study was to establish a novel rapid molecular detection technique for bovine parainfluenza virus parainfluenza type-3 virus (BPIV3) and loop mediated isothermal amplification technique (loop-mediated isothermal amplification LAMPP), and to compare its specificity and sensitivity. The specificity and sensitivity tests showed that the established Nano-PCR and LAMP methods were only specific to BPIV3, but to bovine respiratory syncytial virus and bovine infectious rhinotracheitis virus. There was no cross reaction between bovine viral diarrhea virus (BVDV), and the sensitivity of Nano-PCR and LAMP was the same, which was 10 times of that of normal PCR, and the lowest detection amount of nucleic acid was 4.16 脳 10 ~ 2 copies / 渭 L. The positive coincidence rate of the two methods was 100, and the positive rate was higher than that of normal PCR.Therefore, the Nano-PCR and LAMP methods established in this experiment provide a more rapid, sensitive and reliable tool for the clinical diagnosis of BPIV3.
【作者单位】：东北农业大学;中国农业科学院特产研究所农业部经济动物疫病重点实验室;安图县畜牧兽医总站;
【基金】：国家重点研发计划:畜禽重大疫病防控于高效安全养殖综合技术研发(2016YFD0500907) 吉林省科技厅重点科技成果转化项目(20150307024NY) 吉林省科技厅产业技术创新战略联盟项目(20170309002NY)
【分类号】：S852.653

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