CXCR1基因编码区SNPs与荷斯坦牛泌乳性能和繁殖性状关联分析

发布时间：2018-03-30 07:28

本文选题：荷斯坦牛　切入点：CXCR基因　出处：《东北农业大学学报》2017年11期

【摘要】：研究旨在探索CXCR1(Chemokine(C-X-C motif)receptor 1)基因编码区SNPs与荷斯坦牛泌乳性状及部分繁殖性状间关系。用飞行时间质谱法检测865头荷斯坦牛CXCR1基因编码区c.642 AG、c.816 CA、c.980 AG和c.1068 GA 4个SNPs位点,同时收集采样牛2010年12月至2012年7月繁殖指标和2010年至2014年奶牛生产性能测定(Dairy herd improvement,DHI)记录。采用多因素方差分析法分析CXCR1基因CDS区SNPs基因型与测定日产奶量、乳脂率、乳蛋白率等泌乳性状和产犊间隔、产后首配泌乳天数、配种次数和妊娠泌乳天数关系。结果表明,CXCR1-642 AG和CXCR1-980 AG对乳中体细胞评分(Somatic cell score,SCS)影响显著(P0.05),c.642 AG、c.816 CA、c.980 AG和c.1068 GA对乳中乳脂率、蛋白率和总固体影响显著(P0.05)。CXCR1-980 AG和CXCR1-1068 GA位点AA型个体测定日产奶量显著高于AG和GG型(P0.05),但CXCR1-980 AG AA型乳蛋白率、SCS和总固体显著低于AG和GG型(P0.05)。CXCR1-816 CA位点对产犊间隔影响达显著水平(P=0.033),AC基因型个体产犊间隔显著低于AA型个体。而CXCR1-642 AG、CXCR1-980 AG和CXCR1-1068 GA位点对产犊间隔、产后首配泌乳天数、配种次数以及妊娠泌乳天数均无显著性关联(P0.05)。CXCR1-980 AG和CXCR1-1068 GA对荷斯坦牛产奶量和乳品质存在显著遗传效应,CXCR1 c.816 CA位点对产犊间隔影响显著。CXCR1基因CDS区SNP可作为提高产奶量和乳品质、缩短产犊间隔候选分子标记之一。
[Abstract]:The purpose of this study was to explore the relationship between CXCR1(Chemokine(C-X-C motif)receptor 1 (SNPs) gene coding region and lactating traits and some reproductive traits of Holstein cattle. Four SNPs loci of CXCR1 gene coding region c. 642 AGN c. 816 CAC. 980 AG and c. 1068 GA in 865 Holstein cattle were detected by time-of-flight mass spectrometry. The reproductive indexes of sampled cattle from December 2010 to July 2012 and the Dairy herd provementation data from 2010 to 2014 were collected. Multivariate variance analysis was used to analyze the SNPs genotypes in the CDS region of the CXCR1 gene and the daily milk yield and milk fat rate. The results showed that CXCR1-642 AG and CXCR1-980 AG had a significant effect on milk fat rate of milk by Somatic cell scoreSs, the results showed that the milk fat rate was significantly affected by CXCR1-642 AG and CXCR1-980 AG on the somatic cell score of SCSs, and the results showed that CXCR1-642 AGc.816 CAc.980 AG and c.1068 GA had significant effects on the milk fat rate of milk. Protein rate and total solids were significantly higher than those of AG and GG type P0.05, but the milk protein rates of CXCR1-980 AG and GG type AA milk protein were significantly lower than those of AG and GG type P0.05. CXCR1-816CA loci were significantly lower than those of AG and GG type P0.05. CXCR1-816CA sites. The interval of calving in individuals with AC genotype was significantly lower than that in individuals with AA genotype, while the calving interval was at the sites of CXCR1-642 AGCXCR1-980 AG and CXCR1-1068 GA. Days of first lactation after delivery, There was no significant correlation between the frequency of breeding and the number of days of lactation. There was a significant genetic effect between CXCR1-980 AG and CXCR1-1068 GA on milk yield and quality of Holstein cattle. The effect of CXCR1 c.816CA locus on calving interval was significant. SNP of CDS region of CXCR1 gene could improve milk yield and milk quality. One of the candidate molecular markers for shortening calving interval.
【作者单位】：扬州大学动物科学与技术学院;
【基金】：国家自然科学基金项目(31372286) 江苏省农业自主创新基金(CX(17)1005)
【分类号】：S823

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