羊伪狂犬病毒的分离鉴定

发布时间：2018-03-30 18:50

本文选题：羊　切入点：伪狂犬病毒(PRV)　出处：《黑龙江畜牧兽医》2017年12期

【摘要】：山东省某羊场猪羊混养,羊发生了一种以口吐白沫、有神经症状、腿部部分掉毛为特征的疾病,死亡率高达100%。为确定病原,对病死羊进行病原分离、PCR检测、直接免疫荧光鉴定,确诊病原为伪狂犬病毒(PRV)。通过测序发现,分离毒株的g E基因与NCBI上公布的已知PRV基因组核苷酸序列同源性在97%~99%之间,说明分离株为伪狂犬强毒。该病毒通过BHK细胞系传代至第7代时出现了明显的细胞病变,按照Reed-Muench法计算病毒半数组织细胞感染量(TCID50)为1×107.5/m L。用分离株毒液注射体重20 kg健康绵羊2只,4 d后羊只出现伪狂犬病(PR)的典型症状。根据临床症状及PCR诊断结果,通过采取淘汰场内猪群并对猪舍加强消毒、病羊注射伪狂犬血清、假定健康羊群投喂抗病毒中药七清败毒颗粒等综合防控措施,羊场疫情得到了有效控制。
[Abstract]:In a sheep farm in Shandong Province, when pigs and sheep were mixed, the sheep developed a disease characterized by foaming at the mouth, neurological symptoms, and partial hair loss in the legs. The mortality rate was as high as 100. In order to determine the pathogen, the pathogenic isolation PCR was performed on the dead sheep, and direct immunofluorescence was used to identify the disease. The confirmed pathogen was pseudorabies virus (PRV). By sequencing, the homology of the GE gene of the isolated strain with the known nucleotide sequence of PRV published on NCBI was between 97% and 99%. The results showed that the strain was pseudorabies virulent, and the virus was passed through BHK cell line to the 7th passage with obvious cytopathic effect. TCID50 was 1 脳 107.5 / mL according to Reed-Muench method. The typical symptoms of pseudorabies were found in 2 healthy sheep weighing 20kg after injection with the isolate venom for 4 days. The clinical symptoms and the diagnosis of PCR were analyzed. The epidemic situation in sheep farm was effectively controlled by comprehensive prevention and control measures such as eliminating pig herd and sterilizing pig house, injecting pseudorabies serum into sick sheep, and assuming that healthy sheep were fed with Qiqing Baidu granule, an antiviral medicine.
【作者单位】：山东省滨州畜牧兽医研究院;山东省畜禽用蜂胶疫苗研究开发推广中心;山东绿都生物科技有限公司;
【基金】：山东省现代农业产业技术体系羊创新团队项目(SDAIT-11-16)
【分类号】：S852.654

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