新疆部分地区鸡源沙门氏菌分离鉴定与防治技术研究

发布时间：2018-03-31 04:00

本文选题：新疆　切入点：鸡　出处：《新疆农业大学》2015年硕士论文

【摘要】：沙门氏菌为肠杆菌科中最常见的人畜共患病原菌之一。近年来由于抗生素在兽医临床上的大量应用,导致多重耐药沙门氏菌菌株不断出现,已经给家禽养殖业造成了巨大的经济损失。本文通过对新疆部分地区鸡沙门氏菌进行分离、血清型鉴定、药物敏感性进行研究,为新疆地区鸡沙门氏菌的防治提供理论与实践依据。从伊犁地区、乌鲁木齐市以及周边地区采集肝脏、肛拭子、卵黄液等病料共550份。对所采样品进行增菌、培养、革兰氏染色,初步筛选出疑似为沙门氏菌的细菌;然后进行生化试验鉴定,对确定为沙门氏菌的菌株鉴定其血清型。对鉴定为阳性的沙门氏菌进行药物敏感性实验,筛选出较敏感性的药物,进行临床病例的治疗,并观察临床用药效果。乌鲁木齐市分离的178份病料中,检出8株沙门氏菌,检出率约为4.5%;乌鲁木齐周边212份成年鸡十二指肠内容物和肛拭子中分离出2株沙门氏菌,检出率为0.9%;伊犁地区的160份毛蛋中,检出24株沙门氏菌,检出率为15%;成功分离了34株鸡沙门氏菌,血清群主要为B、C、D三个群,O9有17株,O7型有9株,O4型有7株,O8型有1株,新疆部分地区多发的为O9型沙门氏菌。其中对链霉素、萘啶酸、四环素、磺胺、氨苄西林、呋喃妥因耐药性较高,对头孢噻肟、头孢曲松、头孢他啶、氨曲南、阿莫西林、庆大霉素、复方新诺明、氯霉素等药物具有较高的敏感性。临床治疗选择阿莫西林、环丙沙星饮水,同时配合电解多维、鱼肝油、葡萄糖粉拌料来进行综合治疗鸡沙门氏菌病效果较好。本研究对新疆部分地区的鸡沙门氏菌临床防治提供了理论和实践依据。
[Abstract]:Salmonella is one of the most common zoonotic pathogens in Enterobacteriaceae. In recent years, due to the extensive use of antibiotics in veterinary clinic, multidrug resistant Salmonella strains have been emerging. It has caused enormous economic losses to poultry industry. This paper studied the isolation, serotype identification and drug sensitivity of Salmonella from chickens in some areas of Xinjiang. 550 samples of liver, anal swab and egg yolk fluid were collected from Yili area, Urumqi city and surrounding area. By Gram staining, the bacteria suspected to be Salmonella were preliminarily screened, and then biochemical tests were carried out to identify the serotypes of the strains identified as Salmonella, and the drug sensitivity tests were carried out for the salmonella strains identified as positive. The more sensitive drugs were screened, the clinical cases were treated, and the clinical efficacy was observed. Out of 178 samples isolated from Urumqi, 8 strains of salmonella were detected. The detection rate was about 4.5. Two strains of salmonella were isolated from 212 adult chicken duodenum contents and anal swabs around Urumqi, and the detectable rate was 0.9. In 160 eggs of Yili area, 24 strains of salmonella were detected. The positive rate was 15. 34 strains of Salmonella chickensi were isolated successfully, 17 strains of O7, 9 strains of O4, 7 strains of O8, and 1 strain of O9, among which streptomycin and nalididinic acid, were isolated. High resistance to cefotaxime, ceftriaxone, ceftazidime, aztreonam, amoxicillin, gentamicin, compound sulfamide, nitrofurantoin, tetracycline, sulfamide, ampicillin, furantoin, cefotaxime, ceftriaxone, ceftazidime, Chloramphenicol and other drugs have a high sensitivity. Clinical treatment of amoxicillin ciprofloxacin drinking water, with electrolytic multidimensional, cod liver oil, This study provided theoretical and practical basis for clinical prevention and treatment of chicken salmonella in some areas of Xinjiang.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31

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