甲醛口服液治疗绵羊大肠杆菌性腹泻的效果研究

发布时间：2018-04-02 08:46

本文选题：甲醛　切入点：绵羊　出处：《黑龙江八一农垦大学》2017年硕士论文

【摘要】：由各种原因引起的绵羊腹泻病是当今养羊业发展的巨大障碍。本试验将大庆市周边某羊场的爆发性腹泻病病例作为切入点,研究甲醛口服液对绵羊腹泻病的治疗效果。选择健康绵羊5只灌服甲醛口服液做安全试验;另选健康绵羊5只,腹泻绵羊12只做治疗试验。在治疗试验中选2只腹泻绵羊直接屠宰做病理组织切片检查,剩余10只绵羊,平均分为2组,每组5只,对照组采用羊场常规治疗即肌注恩诺沙星,试验组进行灌服5.6 m L甲醛口服液治疗,健康组正常饲养。三组试验羊于试验期第1 d、2 d、4 d颈静脉采血,检测RBC、HCT、HGB、MCV、WBC、Seg neutr、Band neutr、Mon、Lym、Eos血液生理指标和ALB、ALKP、ALT、GGT、GLU、TP、CREA、BUN血液生化指标;试验期第1 d、2 d、4 d、6d采集绵羊粪便检测性状、寄生虫、白细胞、红细胞、上皮细胞、淀粉颗粒、真菌等指标。试验完毕后,三组中各选择一只绵羊屠宰,取皱胃和十二指肠组织样本,应用HE染色法对其黏膜形态结构、炎性细胞数量变化等进行检测。试验结果:(1)综合绵羊发病情况、临床症状、剖检变化、细菌分离鉴定及动物试验等,确定本次羊场爆发的绵羊腹泻病病原为致病性大肠杆菌。(2)安全试验中,绵羊灌服甲醛口服液后,各项血液生理、生化指标变化不明显,粪便检查未见异常。(3)治疗试验中,试验第1 d,试验组与健康组相比,WBC、Band neutr含量急剧上升且差异极显著(P0.01),治疗后第4 d含量有所降低,差异不显著(P0.05);试验1 d时,试验组和对照组绵羊的Lym较健康组有所降低,其中,试验组与对照组处于同一水平差异不显著(P0.05),而试验组与健康组相比差异显著(P0.05),第4 d时差异不显著(P0.05);试验组绵羊第1 d的MCV与健康组相比较,平均值有所升高且差异极显著(P0.01),与对照组相比差异显著(P0.05),第2 d平均值稍有降低但与健康组相比较差异显著(P0.05),与对照组差异不显著(P0.05),第4 d时,试验组与其他两组比较,差异均不显著(P0.05);生化指标中试验组与健康组比较,GLU含量第1 d和第2 d时有所降低,且差异显著(P0.05),第4 d时差异不显著(P0.05);试验组绵羊血液中TP含量第1 d时与健康组相比较,有所升高且差异显著(P0.05),第2 d、4 d时试验组与其他两组比较差异均不显著(P0.05);与健康组的粪检结果相比较,试验组粪便第4 d时稀便全部转为干便,对照组仍存在稀便且有炎性细胞存在。综上所述,5.6 m L甲醛口服液对健康绵羊无毒害作用,对绵羊大肠杆菌性腹泻有治疗效果,且相同天数时,灌服甲醛口服液的试验组治疗效果优于恩诺沙星治疗的对照组。
[Abstract]:Sheep diarrhea caused by various reasons is a great obstacle to the development of sheep industry.In this study, the effect of formaldehyde oral solution on sheep diarrhea was studied by taking the case of outbreak diarrhea in a sheep farm around Daqing as the cut-in point.5 healthy sheep were given formaldehyde oral solution for safety test, 5 healthy sheep and 12 diarrhea sheep were selected for treatment test.In the treatment experiment, 2 diarrhea sheep were slaughtered directly for histopathological examination. The remaining 10 sheep were divided into 2 groups on average, 5 in each group. The control group was treated with routine treatment of sheep farm, that is, enrofloxacin was injected intramuscularly.The experimental group was treated with 5.6 mL formaldehyde oral solution, and the healthy group was fed normally.Starch granules, fungi and other indicators.After the experiment, one sheep was slaughtered in each group, and the samples of abomasum and duodenum were collected. The morphological structure of mucosa and the number of inflammatory cells were detected by HE staining.Results: (1) synthesizing the incidence, clinical symptoms, dissecting changes, bacteria isolation and identification and animal tests of sheep, confirmed that the pathogen of sheep diarrhea disease in this sheep farm was pathogenic Escherichia coli.After the sheep was given formaldehyde oral liquid, the changes of blood physiological and biochemical indexes were not obvious, and the fecal examination was not abnormal.On the first day of the experiment, the content of neutr in the test group and the control group increased sharply and the difference was very significant (P 0.01). The content of Lym in the test group and the control group was lower than that in the healthy group on the 4th day after treatment, and the Lym of the test group and the control group was lower than that of the healthy group.There was no significant difference between the test group and the control group at the same level (P 0.05), but the difference between the test group and the healthy group was significant (P 0.05), and there was no significant difference at the 4th day (P 0.05). The MCV of the test group was compared with that of the healthy group on the first day.The content of GLU in the test group was lower than that in the healthy group on the 1st and 2nd day, and there was no significant difference between the test group and the healthy group on the 4th day, and the TP content in the blood of the test group was compared with that of the healthy group on the first day.There was no significant difference between the test group and the other two groups on the second day after 4 days, and compared with the results of fecal examination in the healthy group, the feces in the test group turned to dry stool at the 4th day, and there was no significant difference between the two groups on the 4th day after the treatment, compared with the results of fecal examination in the healthy group, the feces in the test group turned to dry stool.In the control group, there were still dilute stool and inflammatory cells.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.26

【参考文献】