陕北白绒山羊10月龄母羊发情周期血清激素分泌规律的研究

发布时间：2018-04-05 10:09

  本文选题：陕北白绒山羊　切入点：卵泡刺激素　出处：《中国畜牧杂志》2017年09期

【摘要】：本实验旨在分析陕北白绒山羊10月龄母羊发情周期内卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E_2)和孕酮(P_4)的含量变化规律,为研究幼龄陕北白绒山羊母羊的繁殖性能和生殖激素的调控提供理论依据。在一个发情周期内,连续20 d每天对8只10月龄陕北白绒山羊母羊进行颈静脉采血(母羊出现发情表现记为发情第0天);在发情期当天和发情间期第5天,从08:00开始,每间隔15 min连续采血3 h,分离血清,用酶联免疫测定法(ELISA)测定发情期、间情期和发情周期血清中FSH、LH、E_2和P_4的含量。结果表明:在发情期和间情期连续3 h内,FSH、LH、E_2和P_4均呈波动式分泌,FSH发情期比间情期波动频率快。LH发情期较间情期的波动频率低;在发情期血清内E_2含量较间情期高(P0.05);间情期血清内P4含量显著高于发情期(P0.05);在测定的20 d内,FSH、LH、E2血清中浓度值差异不显著(P0.05),而P4浓度在发情当日和发情次日低于发情间期(P0.05)。
[Abstract]:The purpose of this experiment was to analyze the changes of follicle stimulating hormone (FSHN), luteinizing hormone (LHH), estradiol (E2) and progesterone (P4) in the estrous cycle of white cashmere goats in Northern Shaanxi.To provide theoretical basis for the study of reproductive performance and regulation of reproductive hormones in young White Cashmere Goat ewes.In a single estrus cycle, 8 10-month-old white cashmere goat estrus were collected by jugular vein every day for 20 consecutive days (estrus appearance in estrus was recorded as the 0th day of estrus, and on the day of estrus and the 5th day of estrus, from 08:00).Serum samples were collected at intervals of 15 min for 3 hours. The serum levels of FSHLHX E2 and PST4 in estrus, meioestrus and estrus were determined by Elisa.鍦ㄥ彂鎯呮湡琛，

本文编号：1714304