postmenopausal osteoporosis animal mode rat ovariectomy

  本文关键词：SD大鼠绝经后骨质疏松疾病动物模型的构建，，由笔耕文化传播整理发布。

SD大鼠绝经后骨质疏松疾病动物模型的构建

An animal model establishment of SD rat with women＇ s postmenopausal osteoporosis

[1] [2] [3]

QI Shan-shan, WANG Yong-ji, XIN Wei （ Vitamin D Research Institute, Shanxi University of Technology, Hanzhong 723000, China）

陕西理工学院维生素D生理与应用研究所,汉中723000

文章摘要：目的 构建绝经后骨质疏松疾病动物模型,探讨该疾病的发病机制与防治措施。方法 将3个月龄健康雌性SD大鼠40只,随机分为2组,分别为去卵巢组和假手术组。去卵巢组切除大鼠双侧卵巢,手术后3个月通过检测体重、阴道涂片、血钙、子宫湿重、骨组织病理学、骨组织形态计量参数等对模型进行评价。结果 手术后3个月,去卵巢组大鼠体重较假手术组明显增加（P〈0.05）,子宫湿重、血钙含量较假手术组量明显下降（P〈0.05）,阴道涂片显示去卵巢组大鼠均无发情表现,骨组织病理学观察显示去卵巢大鼠胫骨骨小梁断裂,小梁间距变宽,结构紊乱,骨形态计量学数据显示去卵巢大鼠胫骨、股骨干骺端骨小梁面积百分率、骨小梁平均厚度均较假手术组减少（P〈0.05）。结论 3个月龄SD大鼠去卵巢后3个月可成功复制出绝经后骨质疏松模型。

Abstr：Purpose To establish an animal model of women＇ s postmenopausal osteoporosis, and to explore the pathogenesis ; the prevention and control measures of the disease. Methods Forty female rats aged 3 months old were divided into ovariectomized group and sham operation group. Bilateral ovaries were removed in the ovariectomized group. The body weight, vaginal smear, serum calcium, wet weight of uterine, and the bone tissue histopathological and morphometrie analysis data were collected at 3 months after operation. Results At 3 months after operation, the body weight were increased in the ovariectomized group compared with the sham operation group and the difference was statistically significant （ P 〈 0.05 ） , the wet weight of uterine and the serum calcium was decreased in the ovariectomized group compared with the sham operation group （ P 〈 0. 05 ）, vaginal smears showed that there were no estrus in the the ovarieetomized group. The hone histopathology results showed that the tihial trahecular fraetured, trabecular spacing widened, and the bone structure was disorder in the ovariectomized group. Morphometfic analysis showed that the area and thickness of the trahecular bone of tibia and femur in the ovariectomized animals were decreased compared with that in the sham operation group （ P 〈 0.05 ）. Conclusion The postmenopausal osteoporosis model can he sueessfully established in 3 months old rats 3 months after ovariectomy operation.

文章关键词：

Keyword：:postmenopausal osteoporosis animal mode rat ovariectomy

课题项目：陕西省科技厅自然科学基础研究项目（2015JQ8305）; 陕西理工学院博士科研启动项目（SLGQD1319）; 陕西省科技创新团队项目（2012KCT29）

  本文关键词：SD大鼠绝经后骨质疏松疾病动物模型的构建，由笔耕文化传播整理发布。