版纳微型猪近交系RNF4基因克

发布时间：2018-04-06 02:31

本文选题：RING指环　切入点：版纳微型猪近交系　出处：《东北农业大学学报》2017年03期

【摘要】：RNF4基因调节精细胞分化和增殖过程,文章以Gen Bank下载的猪及近缘物种RNF4 mRNA序列为参考序列,设计特异引物扩增版纳微型猪近交系(BMI)RNF4基因。应用qPCR分析15个重要组织mRNA表达谱,并对蛋白质序列作功能生物信息学分析,构建多物种系统进化树。研究获得BMI RNF4 573 bp编码区序列(Gen Bank登录号:KU705638,对应氨基酸登录号:AOC89056),编码190个氨基酸,蛋白质分子质量(Mw)为21.43 ku,等电点(pI)为6.95。多组织荧光定量表达分析表明RNF4基因在尿道球腺、睾丸、肝、肺中高水平表达;在其他11个组织中呈中低水平表达。功能生物信息学分析表明RNF4蛋白质存在1个保守结构域,无跨膜结构,无信号肽序列;N末端疏水,C末端疏水;有4类功能活性位点,位于细胞核概率为94.1%。系统进化分析表明,BMI与狗亲缘关系最近。结果为研究RNF4基因在猪精细胞分化和增殖方面作用奠定基础。
[Abstract]:RNF4 gene regulates the process of spermatocyte differentiation and proliferation. A specific primer was designed to amplify the BMI-RNF4 gene of Banna miniature pig inbred line, using the RNF4 mRNA sequence downloaded by Gen Bank as the reference sequence.QPCR was used to analyze mRNA expression profiles of 15 important tissues and functional bioinformatics was used to analyze protein sequences to construct phylogenetic tree of multiple species.The sequence of BMI RNF4 573bp coding region was obtained. The accession number of GenGen Bank was: KU705638, the corresponding amino acid login number was: AOC8905656, encoding 190 amino acids, the molecular weight of protein was 21.43 ku. the isoelectric point (Pi) was 6.95.Fluorescence quantitative analysis showed that the expression of RNF4 gene was high in urethral glandular gland, testis, liver and lung, and low in other 11 tissues.Functional bioinformatics analysis showed that RNF4 protein had a conserved domain, no transmembrane structure and no signal peptide sequence.Phylogenetic analysis showed that BMI had the closest relationship with dogs.Results the results laid a foundation for the study of the role of RNF4 gene in the differentiation and proliferation of porcine spermatocytes.
【作者单位】：云南农业大学云南省版纳微型猪近交系重点实验室;云南农业大学动物科学技术学院;
【基金】：国家自然科学基金项目(31660637,31460580,31660650)
【分类号】：S828

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