不同载体预混合饲料中维生素E近红外光谱模型

发布时间：2018-04-08 07:08

本文选题：载体稀释剂　切入点：维生素E　出处：《中国农业科学》2017年20期

【摘要】：【目的】维生素E是重要饲料添加剂,其含量的准确测定对饲粮配制具有重要意义。文章探讨了不同载体稀释剂预混合饲料中维生素E的快速定量方法,旨在说明近红外光谱分析方法对不同载体预混合饲料的适用性,对日后建立预混合饲料中维生素含量的近红外速测方法提供参考。【方法】采集商品维生素E粉剂,以市场上常见的二氧化硅、脱脂米糠和石粉为载体稀释剂,通过混匀机混匀配制了浓度范围为5—250 IU·g~(-1)的维生素E预混合饲料,并采集其近红外光谱。研究比较了不同扫描次数和分辨率对近红外光谱质量的影响,比较了不同载体稀释剂预混合饲料中维生素E的特征峰及不同光谱预处理方式下维生素E偏最小二乘回归模型的定量精度,并从PLS模型中的VIP Score值的角度来分析模型的差异性。【结果】16 cm~(-1)分辨率和32次扫描次数为适宜的光谱采集参数;不同载体稀释剂预混合饲料的近红外光谱差异明显,二氧化硅、脱脂米糠和石粉为载体的预混合饲料维生素E主要特征吸收峰不同,分别位于8 700—8 200、7 100—7 450、6 020—5 550、4 440—4 200、5 210—4 760,8 550—8 050、7 410、5 950—5 200、4 400—4 050、4 750—4 600 cm-1和8 550—8 050、7 250、5 950—5 200、4 600—4 000、4 800—4 700 cm~(-1)其近红外定量模型的最佳光谱预处理方法分别为SNV+1st Deriv.(2,15)、MSC+1st Deriv(2,21)和SNV+2st Deriv(2,15),其校正决定系数
[Abstract]:[objective] Vitamin E is an important feed additive.The rapid quantitative method of vitamin E in feed premixed with different carrier diluents is discussed in this paper. The purpose of this paper is to explain the applicability of near infrared spectroscopy analysis method to feed premixed with different carriers.It provides a reference for the establishment of the near infrared rapid determination method of vitamin content in premixed feed. [methods] the commercial vitamin E powder was collected, and the common silica, defatted rice bran and stone powder were used as carrier diluents.Vitamin E premixed feed with concentration range of 5-250 IU / g ~ (-1) was prepared by mixing machine and its near infrared spectrum was collected.The effects of different scanning times and resolutions on the quality of NIR spectra were studied and compared.The characteristic peaks of vitamin E in different carrier diluents premixed feed and the quantitative accuracy of partial least square regression model of vitamin E in different spectral pretreatment methods were compared.From the point of view of VIP Score value in the PLS model, the difference of the model was analyzed. [results] the resolution of 16 cm ~ (-1) and the number of 32 times of scanning were the appropriate spectral acquisition parameters, and the near infrared spectra of different diluents premixed feed with different carriers were obviously different.The absorption peaks of vitamin E in premixed feed with silica, defatted rice bran and stone powder as carriers are different.The optimum spectral pretreatment methods of SNV 1st Deriv.Derivlv.HY21 and SNV 2st Derivor 2J are respectively. The calibration decision coefficients are obtained.
【作者单位】：中国农业科学院农业质量标准与检测技术研究所;辽宁省兽药饲料畜产品质量安全检测中心;铂金埃尔默企业管理(上海)有限公司;
【基金】：“十三五”国家重点研发计划(2016YFF0201802) 中国农业科学院基本科研业务费专项(1610072017001);中国农业科学院“饲料质量安全检测与评价”创新团队项目资助
【分类号】：S816

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