抑制素对绵羊颗粒细胞雌激素和孕酮分泌及相关基因表达的影响

发布时间：2018-04-08 11:15

本文选题：抑制素　切入点：雌激素　出处：《畜牧兽医学报》2017年09期

【摘要】：本试验旨在研究RNA干扰抑制素α亚基(Inhibinα-subunit,INHα)基因和添加抑制素A(InhibinA)对绵羊颗粒细胞雌激素(Estrogen,E2)和孕酮(Progesterone,P)分泌及相关基因表达的影响,以探索抑制素在绵羊颗粒细胞E2和P分泌中的作用。从1.0~1.5岁小尾寒羊的卵泡(3~7mm)中分离培养颗粒细胞,分为2个处理组:干扰组(脂质体介导法转染颗粒细胞)和InhibinA添加组(200ng·mL~(-1))。利用ELISA试剂盒检测E2和P的分泌,荧光定量RT-PCR检测E2和P的分泌相关基因(CYP11、3β-HSD和CYP19)的表达量。结果表明,与对照组相比,干扰组siRNA转染颗粒细胞48h后,INHα基因的抑制率达87%,E2和P的分泌量显著降低(P0.05);InhibinA添加组E2和P的分泌水平显著升高(P0.05);干扰组3β-HSD和CYP19的mRNA表达量显著降低(P0.05),CYP11的表达量显著升高(P0.05);InhibinA添加组CYP19、CYP11和3β-HSD的mRNA表达水平均显著升高(P0.05)。综上表明,抑制素在绵羊颗粒细胞中起关键调控作用,通过调节绵羊颗粒细胞类固醇激素的分泌参与调控卵泡发育和排卵过程。
[Abstract]:The aim of this study was to investigate the effects of RNA interference inhibitor 伪 -subunit INH 伪) gene and the addition of inhibin A on the secretion of estradiol (Estrogen2) and progesterone (P) in sheep granulosa cells, and to explore the role of inhibin in the secretion of E2 and P in sheep granulosa cells.Granulosa cells were isolated from 1. 0 and 1.5 year old small tail Han sheep. They were divided into two groups: interference group (transfection of granulosa cells by liposome) and InhibinA addition group (200ng / mL).The secretion of E2 and P was detected by ELISA kit and the expression of CYP113 尾 -HSD and CYP19 was detected by fluorescence quantitative RT-PCR.The results showed that, compared with the control group,The mRNA expression levels of CYP19, CYP11 and 3 尾 -HSD were significantly increased in the addition group of P0.05InhibinA, and the expression levels of CYP11 and 3 尾 -HSD were significantly increased.In conclusion, inhibin plays a key role in the regulation of sheep granulosa cells, by regulating the secretion of steroid hormones in sheep granulosa cells to regulate follicular development and ovulation.
【作者单位】：河北农业大学动物科技学院;
【基金】：国家现代肉羊产业技术体系项目(CARS-39)
【分类号】：S826

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