高通量测序技术分析新疆新源县过度放牧土壤细菌多样性

发布时间：2018-04-09 10:35

本文选题：高通量测序　切入点：退化　出处：《微生物学通报》2017年03期

【摘要】：【目的】利用新疆伊犁州新源县过度放牧导致的退化草原土壤样品,揭示其细菌的菌群多样性。【方法】通过Illumina Hi Seq 2500测序技术,分析6个退化草原土壤细菌的菌群结构及多样性。【结果】细菌群落Chao1指数平均值为2 118.28,Shannon指数平均值为8.84。细菌群落主要属于Actinobacteria、Proteobacteria、Firmicutes、Gemmatimonadetes等35个门,Phingomonadaceae、Gemmatimonadaceae等220个科,Sphingomonas、Rubrobacter等329个属,在科属水平上未知菌类分别占31%和56.1%。固氮细菌共有9个属,自生固氮菌中Arthrobacter和Methylobacterium的丰度最高,而根瘤菌中Bradyrhizobium和Rhizobacter的丰度较高。【结论】虽然6个退化草原土壤样品中细菌类群具有丰富的多样性,但很多种类丰度特别低,可能正趋向灭绝。
[Abstract]:[objective] to reveal the bacterial diversity of degraded grassland soil samples caused by overgrazing in Xinyuan County, Yili Prefecture, Xinjiang. [methods] Illumina Hi Seq 2500 sequencing technique was used.The structure and diversity of bacteria in 6 degraded steppe soils were analyzed. [results] the average Chao1 index of bacterial community was 2118.28 Shannon index and 8.84.The bacterial community mainly belongs to 329 genera of Sphingomonasaurus Rubrobacter, such as Actinobacteriae Proteobacteriae, first Gemmatimonadetes and other 35 phylum phylum, such as Gemmatimonadaceae, etc. At the level of family and genus, 31% and 56.1% of the unknown fungi are found in the family and genus level, respectively.There were 9 genera of nitrogen-fixing bacteria, and the abundance of Arthrobacter and Methylobacterium was the highest among the autobiotic nitrogen fixing bacteria, while the abundance of Bradyrhizobium and Rhizobacter in rhizobia was higher. [conclusion] although the bacterial groups in 6 degraded grassland soil samples are rich in diversity,But many species are particularly low in abundance and may be heading towards extinction.
【作者单位】：伊犁师范学院生物与地理科学学院;南开大学生命科学学院微生物系;
【基金】：伊犁师范学院一般科研项目(No.2013YSYB15)~~
【分类号】：S812.2

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