肉鸡鸡胚肝脏中脂滴的发育研究

发布时间：2018-04-09 20:30

  本文选题：肉鸡鸡胚　切入点：脂滴　出处：《中国畜牧兽医》2017年03期

【摘要】：为了探索鸡胚肝脏脂滴的发育规律,选取120枚受精蛋,分别在孵化的第7~21天(E7~E21)每天随机选取6枚蛋,收集鸡胚,采集鸡胚肝脏,采用冰冻切片、苏丹Ⅲ染色观察鸡胚肝脏脂滴的形成和发育特点。结果发现:1鸡胚发育到E9胚龄,肝细胞内开始出现脂滴,且随着胚龄增加,脂滴的数量不断增多,脂滴的大小不断增大;2E18胚龄以前,肝脏脂滴的分布从中央静脉开始沿肝索到肝小叶边缘数量逐渐减少,E18胚龄以后,肝脏边缘脂滴数量较肝脏中央的多;3肝脏脂滴的发育分为E9~E14、E15~E17和E18~E21 3个阶段,而且后一个阶段脂滴的数量和体积均显著大于前一个阶段(P0.05),脂滴的平均大小在E9~E14、E15~E17和E18~E21 3个阶段分别为3.2、5.7和10.8μm,平均数量分别为67、327、397个/mm2。以上结果表明,随着鸡胚的发育,肝细胞内脂滴数量增加、体积变大。
[Abstract]:In order to explore the development rule of lipid droplets in the liver of chicken embryo, 120 fertilized eggs were selected, 6 eggs were randomly selected every day on the 7th 21st day of hatching. Chicken embryo was collected, liver of chicken embryo was collected and frozen section was used.The formation and development of lipid droplets in the liver of chicken embryo were observed by Sudan 鈪，

本文编号：1728028