绵羊产肉性能分子标记筛选

发布时间：2018-04-11 18:35

本文选题：绵羊 + 产肉性能　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：绵羊产肉性能主要包括生长性状、繁殖性状和肉质性状。肉羊养殖以出栏早、投入小、周转快等特点受到牧民和牧场主的青睐,同时羊肉以其肉质鲜美、低脂肪、低胆固醇等特点深受消费者的喜爱。因此,提高肉用绵羊产肉性能迫在眉睫。本研究结合转录组、基因组和表观遗传学等多种组学方法,深入挖掘了绵羊产肉性能相关的分子标记。利用转录组分析方法挖掘绵羊增重性状相关分子标记;利用全基因组重测序技术筛选与绵羊繁殖性状相关分子标记;利用MeDIP-Seq测序技术,从表观遗传学方向挖掘地域差异下影响绵羊肉质性状相关分子标记。主要研究结果如下:(1)通过血液转录组分析方法结合表型数据,筛选出135个与绵羊增重性状相关分子标记;(2)通过基因组重测序方法,筛选得到107个与绵羊繁殖性状相关分子标记;(3)基于MeDIP-seq测序数据挖掘出222个与绵羊肉质性状相关分子标记;将筛选出的464个分子标记设计出绵羊产肉性能相关液相芯片。通过上述研究方法为筛选出绵羊产肉性能相关的分子标记并设计相关探针。这为绵羊分子育种提供了一种新的思路和方法;同时,也为实现绵羊经济性状的早期选育,提高绵羊产肉性能提供了新的方向。
[Abstract]:The meat yield of sheep mainly includes growth traits, breeding traits and meat quality traits.Meat sheep breeding is favored by herdsmen and ranchers for its characteristics of early production, small investment and quick turnover, while mutton is loved by consumers for its delicious meat, low fat, low cholesterol and so on.Therefore, it is urgent to improve the meat production performance of meat sheep.Combined with transcriptome, genome and epigenetics, the molecular markers related to sheep meat production were excavated.Molecular markers related to sheep weight gain traits were excavated by transcriptome analysis, molecular markers associated with sheep reproductive traits were screened by whole genome resequencing technique, and MeDIP-Seq sequencing techniques were used.The related molecular markers of meat quality traits of sheep were excavated from epigenetics.The main results were as follows: 1) 135 molecular markers associated with sheep weight gain traits were screened by blood transcriptome analysis combined with phenotypic data.107 molecular markers related to sheep reproductive traits were obtained. 222 molecular markers related to sheep meat quality traits were mined based on MeDIP-seq sequencing data.The above methods were used to screen out the molecular markers related to sheep meat production and to design the relevant probes.This provides a new way of thinking and method for sheep molecular breeding, and also provides a new direction for the early breeding of sheep economic traits and the improvement of sheep meat production performance.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S826

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