中药复方多糖对鸡淋巴细胞免疫信号分子表达的影响

发布时间：2018-04-11 19:48

本文选题：中药复方 + 多糖　；参考：《江苏农业科学》2017年05期

【摘要】：探讨在体外环境下不同质量浓度中药复方多糖对鸡外周血淋巴细胞信号分子表达的影响。分别采用200、100、50、25、0μg/m L 5个中药复方多糖质量浓度梯度在体外条件下刺激鸡外周血淋巴细胞,采用ELISA法测定中药多糖作用1、2、4 h时细胞内环磷酸腺苷(cAMP)、环磷酸鸟苷(c GMP)的含量,以及作用24 h时钙离子(Ca2+)、一氧化氮(NO)、一氧化氮合成酶(i NOS)的分泌水平。结果表明,与对照组相比,试验组能显著提高细胞培养上清中cAMP、c GMP的水平(P0.05)。当作用1 h时,100μg/m L多糖处理组与其他试验组的cAMP差异显著(P0.05);作用2、4 h时,200μg/m L多糖组的cAMP、c GMP分泌水平显著高于其他试验组。与对照组相比,100、200μg/m L多糖组能显著提高细胞培养上清液中Ca2+、NO的分泌水平(P0.05),25、50μg/m L多糖组对淋巴细胞中Ca2+、NO的分泌水平无显著影响(P0.05)。中药复方多糖能显著提高淋巴细胞分泌i NOS的水平(P0.05),而与其他多糖组相比,100μg/m L多糖组淋巴细胞分泌i NOS的水平显著提高(P0.05)。中药复方多糖可通过改变Ca2+、cAMP、c GMP、NO、i NOS等细胞内信号分子的活性或含量而启动细胞信号传导,从而调节细胞的活性和功能,促进相关基因的表达和释放,发挥免疫调节作用。
[Abstract]:To investigate the effects of different concentrations of polysaccharides in vitro on the expression of signal molecules in peripheral blood lymphocytes of chicken.Chicken peripheral blood lymphocytes were stimulated with five Chinese medicine polysaccharides (50 渭 g / mL) in vitro. The contents of intracellular cyclic adenosine monophosphate (camp) and cyclic guanosine monophosphate (cGMP) were determined by ELISA method.The levels of Ca ~ (2 +) ~ (2 +) Ca ~ (2 +) ~ (2 +), no ~ (2 +) ~ (2 +), nitric oxide synthase (iNOS) at 24 h.The results showed that compared with the control group, the level of cAMPC GMP in the culture supernatant of the experimental group was significantly higher than that in the control group (P 0.05).The difference of cAMP between the 100 渭 g / mL polysaccharide treatment group and other experimental groups was significant at 1 h, and the level of cAMPP C GMP secretion in 200 渭 g / mL polysaccharide group was significantly higher than that in other experimental groups at 2 渭 g / mL for 4 h.Compared with the control group, 100200 渭 g / mL polysaccharide group could significantly increase the level of Ca2 no secretion in the supernatant of cell culture.The level of I NOS secreted by lymphocytes was significantly increased by Chinese herbal compound polysaccharides, while the level of I NOS secreted by lymphocytes in 100 渭 g / mL polysaccharides group was significantly higher than that in other polysaccharides groups.The polysaccharides of traditional Chinese medicine can activate the signal transduction by changing the activity or content of signal molecules such as Ca2 cAMPC GMPC NOS and so on, thus regulating the cell activity and function, promoting the expression and release of related genes, and exerting the role of immune regulation.
【作者单位】：石河子大学动物科技学院;
【基金】：国家自然科学基金(编号:31460673)
【分类号】：S853.7

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