半抗原-琼脂扩散试验鉴别布鲁氏菌感染抗体和S2、Rev.1免疫抗体的研究与应用

发布时间：2018-04-13 00:03

本文选题：布鲁氏菌病 + 半抗原-琼脂扩散试验　；参考：《中国人兽共患病学报》2017年02期

【摘要】：目的布鲁氏菌病严重威胁着我国畜牧业生产和公共卫生安全,现有的常规血清学检测方法虽具有较高的敏感性与特异性,但无法区分布鲁氏菌感染抗体和S2、Rev.1疫苗免疫抗体(以下简称"鉴别诊断"),给我国执行扑杀布病感染动物的政策带来阻力。因此在S2和Rev.1疫苗免疫背景下研究天然半抗原-琼脂扩散试验(NH-GD)的鉴别诊断能力。方法优化NH-GD试验条件,测定其敏感性、特异性、重复性及鉴别诊断能力,并与荧光偏振测定法(FPA)作比较。结果 NH-GD试验具有很好的特异性和重复性,对S2免疫羊群从疫苗接种开始就具有鉴别诊断能力,而对Rev.1免疫羊群从疫苗接种122d开始具有鉴别诊断能力;与FPA作比较,一致性较好,且操作技术和所需仪器都非常简单,特别适合在基层推广使用,为我国羊布病监测提供了一个很好的检测方法。
[Abstract]:Objective Brucellosis is a serious threat to the production of animal husbandry and public health in China.However, it is impossible to distinguish the brucellosis infection antibody from the immune antibody against S2GV vaccine (hereinafter referred to as "differential diagnosis"), which brings resistance to the execution of the policy of killing the infected animals of brucellosis in our country.Therefore, the differential diagnosis ability of natural hapten-Agar diffusion test (NH-GD) was studied in the context of S2 and Rev.1 vaccine immunization.Methods the sensitivity, specificity, repeatability and differential diagnostic ability of NH-GD were optimized and compared with that of fluorescence polarization method.Results NH-GD test had good specificity and reproducibility, and had the ability of differential diagnosis for S 2 immunized sheep from vaccination, and for Rev.1 immunized sheep from 122 days after inoculation, compared with FPA.The consistency is good, and the operation technology and the required instruments are very simple, especially suitable for the basic level, which provides a good detection method for sheep cloth disease monitoring in China.
【作者单位】：中国动物卫生与流行病学中心;
【基金】：现代农业(奶牛)产业技术体系建设项目专项经费(CARS-37)资助~~
【分类号】：S855.12

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