上海市猪源多杀性巴氏杆菌分离鉴定及其耐药性分析

发布时间：2018-04-13 02:23

本文选题：多杀性巴氏杆菌 + 分离　；参考：《中国动物传染病学报》2016年06期

【摘要】：本研究旨在调查上海市猪源多杀性巴氏杆菌对临床常用抗菌药的耐药情况,为该病的药物预防与治疗提供科学依据。2014~2015年在上海市11个规模化养猪场采集疑似多杀性巴氏杆菌感染的病料共计116份,采用细菌分离培养及PCR方法,分离鉴定出20株多杀性巴氏杆菌。采用纸片法测定了分离菌株对20种临床常用抗菌药的敏感性,结果表明,所有菌株对阿莫西林克拉维酸、头孢曲松、恩诺沙星、氟苯尼考、阿奇霉素和多黏菌素B敏感,这6种药物可作为本地区控制本病的首选药物。然而,超过50%的菌株对青霉素(65.0%)、复方新诺明(65.0%)、氨苄西林(60.0%)、链霉素(60.0%)、四环素(60.0%)耐药。耐药谱分析显示,85.0%的菌株对4~7种抗菌药耐药。本研究结果表明上海市猪源多杀性巴氏杆菌对临床常用抗菌药物耐药较严重,应加强养殖场猪多杀性巴氏杆菌的耐药性监测与防控。
[Abstract]:The purpose of this study was to investigate the resistance of porcine Pasteurella multocida to antimicrobial agents commonly used in clinic in Shanghai.From 2014 to 2015, 116 samples of suspected Pasteurella multocida infection were collected from 11 large-scale pig farms in Shanghai. Bacterial isolation and culture and PCR method were used.Twenty strains of Pasteurella multocida were isolated and identified.The susceptibility of the isolated strains to 20 commonly used clinical antimicrobial agents was determined by disk method. The results showed that all strains were sensitive to amoxicillin clavulanic acid, ceftriaxone, enrofloxacin, florfenicol, azithromycin and polymyxin B.These 6 kinds of drugs can be used as the first choice to control the disease in this area.However, more than 50% of the strains were resistant to penicillin 65.0, compound sulfamethoxine 65.0, ampicillin 60. 0, streptomycin 60. 0 and tetracycline 60. 0).Resistance spectrum analysis showed that 85.0% of the strains were resistant to 47 antimicrobial agents.The results showed that the resistance of Pasteurella multocida to antimicrobial agents was more serious in Shanghai, and the surveillance and control of drug resistance of Pasteurella multocida should be strengthened.
【作者单位】：湖南省冷水江市畜牧水产局;上海农林职业技术学院;中国农业科学院上海兽医研究所;上海市闵行区动物疫病预防控制中心;
【基金】：上海市生猪产业技术体系建设项目(沪农科产字2015第6号) 上海市科技兴农重点攻关项目(沪农科攻字(2013)第5-6号)
【分类号】：S852.612

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