规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的调查及共转移研究

发布时间：2018-04-15 04:35

本文选题：鸡场 + 大肠杆菌　；参考：《四川大学学报(自然科学版)》2017年02期

【摘要】：调查规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的流行现状及共转移情况,对549株来源于鸡场粪样和苍蝇的大肠杆菌进行blaCMY-2PCR检测;K-B法检测耐药表型;PCR扩增19种相关耐药基因和14种毒力基因;接合试验和质粒复制子分型研究耐药基因与毒力基因的共转移.结果显示:6.0%(33/549)大肠杆菌呈CMY-2阳性,且均为多重耐药;检测到15种耐药基因和4种毒力基因,其中floR,blaTEM-1,sul2,sul1,traT,VagC检出率较高;63.6%产CMY-2大肠杆菌中blaCMY-2基因可通过lncA/C或lncI1质粒与耐药基因和(或)毒力基因共转移.本研究表明规模化鸡场粪样和苍蝇源产CMY-2大肠杆菌已成为耐药基因和毒力基因的重要储存库,携带二者质粒的转移导致耐药与毒力的传播扩散.
[Abstract]:To investigate the prevalence and cotransfer of drug-resistant and virulence genes of CMY-2 Escherichia coli in large-scale chicken farms.19 genes and 14 virulence genes of Escherichia coli from chicken farm feces and flies were detected by blaCMY-2PCR and K-B method.The cotransfer of drug resistance gene and virulence gene was studied by conjugation test and plasmid replicator typing.The results showed that Escherichia coli was CMY-2 positive and multidrug resistant, and 15 resistant genes and 4 virulence genes were detected.Among them, the detection rate of VagC in flor blaTEM-1sul2sul2sul1traTagC was 63.6%, and the blaCMY-2 gene could be co-transferred by lncA/C or lncI1 plasmid with drug resistance gene and / or virulence gene.This study showed that CMY-2 Escherichia coli (E. coli) produced from feces and flies from large scale chicken farms had become an important repository of drug resistance genes and virulence genes. The transfer of the two plasmids led to the spread of drug resistance and virulence.
【作者单位】：四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室生物资源与生态环境教育部重点实验室;
【基金】：国家蛋鸡产业技术体系岗位科学家项目(CARS-41-K09) 国家重点基础研究计划(2013CB127200)
【分类号】：S852.61

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