PRRS疫苗免疫猪群的PRRS病原与抗体检测

发布时间：2018-04-16 03:21

本文选题：PRRS + 免疫猪群　；参考：《金华职业技术学院学报》2016年06期

【摘要】：设计一对能区分经典株与变异株的PRRSV特异性引物,建立PRRSV经典株与变异株的鉴别诊断方法,用该方法对2013年实验室收集到的来自131个免疫PRRS弱毒疫苗猪场的493份临床送检样品进行RT-PCR检测。PRRSV的检出率为27.2%(134/493),其中变异株的检出率为23.1%(114/493),经典株的检出率为8.11%(40/493),20份样品可以同时检出PRRSV变异株和经典株,检出率为4.1%(20/493)。对临床送检的30个免疫PRRS活疫苗猪场的62个猪群1 079份血清,用IDEXX PRRS X3抗体检测试剂盒进行了血清学分析,结果显示总体阳性率为91.01%(982/1079),育肥猪群阳性率最高,达95.26%;其次为母猪群,阳性率高达93.7%;保育猪相对较低,为82.72%。根据猪群临床症状和PRRS状况将猪群分为四种类型。对62个免疫PRRS弱毒疫苗的规模化猪场进行了抗体分析,PRRSV抗体的离散度超过40%的猪场达59.7%(37/62)。这些结果说明,变异株为主要流行毒株,同时存在经典株与变异株的混合感染;免疫猪群抗体不整齐,目前弱毒疫苗免疫效果不理想。
[Abstract]:A pair of PRRSV specific primers were designed to distinguish classical strains from variant strains, and a differential diagnosis method for PRRSV classical strains and variant strains was established.This method was used to detect 493 clinical samples collected from 131 pig farms immunized with PRRS attenuated vaccine in 2013. The detection rate of PRRSv was 27.2% 134 / 493, of which the detection rate of variant strain was 23.1% and that of classical strain was 8.11% -4933%.Both PRRSV variants and classical strains could be detected.The detection rate was 4. 1% 20 / 493%.A serological analysis of 1 079 sera from 62 pigs from 30 live immunized PRRS vaccine farms was carried out by using the IDEXX PRRS X3 antibody test kit. The results showed that the overall positive rate was 91. 01% 982 / 1079%, the positive rate of fattening pigs was the highest (95.26%), and the next was sows.The positive rate was 93. 7% and that of the preserved pig was 82.72%.Pigs were divided into four types according to their clinical symptoms and PRRS status.The antibody analysis of 62 pig farms immunized with PRRS attenuated vaccine was carried out. The number of pig farms where the dispersion of PRRSv antibody was more than 40% was 59.7 / 62.These results indicated that the mutant strain was the main epidemic strain, and there was a mixed infection of classical strain and variant strain, and the antibody of immunized swine group was not neat, so the immune effect of attenuated vaccine was not ideal at present.
【作者单位】：金华职业技术学院;浙江大学农业部动物病毒学重点实验室;浙江同点生物科技有限公司;
【基金】：浙江省科技厅公益技术研究农业项目(2012C22051) 金华市农业类重点研究项目(2014-2-003)
【分类号】：S858.28

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