东北虎源蛔虫线粒体cox1基因序列进化分析研究

发布时间：2018-04-16 14:28

本文选题：蛔虫 + 细胞色素I　；参考：《中国预防兽医学报》2017年01期

【摘要】：为了解东北虎源蛔虫与其他蛔虫的种群遗传关系,本研究对湖南省长沙生态动物园东北虎粪便中采集的蛔虫成虫的线粒体DNA中细胞色素I(cox1)基因序列进行扩增,将测序结果与GenB ank中登录的其他蛔虫cox1序列进行对比及种系发育树分析。结果显示,来自长沙生态动物园7个东北虎蛔虫样品cox1序列长度基本一致,为413 bp~414 bp,与猫弓首蛔虫同源性最高,达98.4%以上,而且系统进化分析显示所有样品均与猫弓首蛔虫位于同一分支。该结果表明,pcox1序列种内相对保守,种间差异较大,因此可以作为猫科弓首蛔虫的种间遗传变异研究的分子遗传标记,从而为蛔虫的群体遗传研究奠定了基础。
[Abstract]:In order to understand the genetic relationship between Ascaris ricoides and other Ascaris, we amplified the cytochrome Icox1 gene from mitochondrial DNA of adult Ascaris lumbricoides collected from the feces of Amur tiger in Changsha Ecological Zoo of Hunan Province.The sequencing results were compared with the cox1 sequences of other Ascaris lumbricoides registered in GenB ank and the phylogenetic tree analysis.The results showed that the length of cox1 sequence of 7 Amur tiger ascariasis samples from Changsha Ecological Zoo was basically the same, which was 413 bp~414 bp. the homology was the highest with Toxocariasis catnii, which was above 98.4%.Phylogenetic analysis showed that all samples were located in the same branch as Ascaris catacaroides.The results showed that the pcox1 sequence was relatively conserved within species and varied greatly among species, so it could be used as a molecular genetic marker for the study of genetic variation of Ascaris lumbricoides among species, thus laying a foundation for the population genetic study of Ascaris lumbricoides.
【作者单位】：长沙生态动物园;湖南农业大学动物医学院;铜陵动物园;
【基金】：湖南省畜牧局项目资助
【分类号】：S852.7

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