茶皂素对奶牛免疫功能的影响

发布时间：2018-04-16 15:30

本文选题：茶皂素 + 免疫　；参考：《动物营养学报》2017年03期

【摘要】：本试验旨在研究茶皂素对荷斯坦奶牛的血清免疫相关细胞因子含量及其mRNA表达量的影响。试验采用4×4拉丁方设计,选取4头瘤胃瘘管奶牛,每头奶牛分别灌注0(对照)、15、30、45 g/d的茶皂素,预试期14 d,正试期21 d,共4期。采用酶联免疫吸附剂测定(ELISA)法试剂盒测定血清中免疫球蛋白(Ig)、白细胞介素(IL)和肿瘤坏死因子-α(TNF-α)的含量,采用实时荧光定量PCR方法测定淋巴细胞IL-1、IL-6、IL-10和TNF-αmRNA表达量。结果显示:1)30 g/d茶皂素组奶牛血清Ig M含量显著高于对照组(P0.05)。2)15 g/d茶皂素组奶牛血清IL-1含量显著高于对照组和45 g/d茶皂素组(P0.05)。3)与对照组相比,15、30、45 g/d茶皂素组奶牛血清IL-1、IL-6、IL-10、TNF-αmRNA表达量均无显著差异(P0.05),但45 g/d茶皂素组可提高以上细胞因子mRNA表达量。由此可见,茶皂素可提高奶牛血清中Ig及免疫相关细胞因子含量,并提高细胞因子IL-6 mRNA表达量,表明茶皂素可提高奶牛免疫功能。
[Abstract]:The aim of this study was to study the effect of tea saponin on serum immune-associated cytokines and mRNA expression in Holstein cows.Elisa kit was used to detect the contents of IgA, IL) and TNF- 伪 in serum, and the expression of IL-6 IL-10 and TNF- 伪 mRNA in lymphocytes was measured by real-time fluorescence quantitative PCR.The results showed that the serum level of IgM in the 30 g / d tea saponin group was significantly higher than that in the control group (P0.05. 2g / d) and the serum IL-1 level in the tea saponin group was significantly higher than that in the control group and the 45 g / d tea saponin group (P < 0.05). Compared with the control group, the serum levels of IL-1-IL-6 IL-10 and TNF- 伪 mRNA in the 30 ~ (g / d) d tea saponin group were significantly higher than those in the control group.However, 45 g / d tea saponin group could increase the mRNA expression of above cytokines.It can be seen that tea saponin can increase the content of Ig and immune-related cytokines in serum and the expression of cytokine IL-6 mRNA, which indicates that tea saponin can improve the immune function of dairy cattle.
【作者单位】：北京农学院动物科学技术学院奶牛营养学北京市重点实验室;中国农业科学院北京畜牧兽医研究所;
【基金】：“十二五”国家科技支撑项目(25012BAD14B09) “十三五”国家重大科技专项(2016YFDO70020,2016YFDO700205) 北京市农业局北京市现代农业产业技术体系奶牛创新团队项目
【分类号】：S823.5

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