猪繁殖与呼吸综合征病毒抗体的荧光微球免疫学检测方法的建立

发布时间：2018-04-16 18:23

本文选题：猪繁殖与呼吸综合征病毒 + 非结构蛋白　；参考：《中国兽医科学》2017年12期

【摘要】：为了建立一种高通量、重复性好、特异性高的猪繁殖与呼吸综合征病毒(PRRSV)抗体的快速检测方法,本研究用纯化后的PRRSV非结构蛋白7(Nsp7)重组蛋白作为偶联抗原建立PRRSV抗体的荧光微球免疫学检测方法。对检测方法进行分析结果表明,批内、批间变异系数分别为3.2%、4.2%,与其他常见猪病阳性血清无交叉反应。当单抗浓度低至1 ng/mL时,该方法仍具有高灵敏度。本研究建立的荧光微球免疫学检测方法能快速检测临床样本PRRSV抗体,为PRRSV检测提供了一种新方法。
[Abstract]:To establish a rapid detection method for PRRSVV antibody of porcine reproductive and respiratory syndrome virus (PRRSVV) with high throughput, good reproducibility and specificity.In this study, a fluorescent microsphere immunological assay for the establishment of PRRSV antibodies using purified PRRSV nonstructural protein 7nsp7) as a conjugated antigen was carried out.The results showed that the coefficient of variation between batches was 3.2 and 4.2, and there was no cross reaction with other common swine disease positive sera.The method still has high sensitivity when the concentration of McAb is as low as 1 ng/mL.The fluorescent microsphere immunoassay developed in this study can quickly detect PRRSV antibodies in clinical samples and provides a new method for PRRSV detection.
【作者单位】：华南农业大学兽医学院;广东省兽医临床重大疾病综合防控重点实验室;
【基金】：国家重点研发计划项目(2016YFD0500707) 广东省高等学校优秀青年教师培养计划项目(YQ2015030)
【分类号】：S852.651

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