富锌益生菌对蛋鸡抗氧化机能及抗病能力的影响

发布时间：2018-04-17 04:01

  本文选题：富锌益生菌 + 蛋鸡　； 参考：《河南农业科学》2017年11期

【摘要】：为研究有益菌转化无机锌制备的富锌益生菌在蛋鸡生产中的应用效果,选取120只19周龄海兰褐蛋鸡,分为对照组和3个试验组,对照组饲喂基础日粮,试验组分别在基础日粮中添加0.10%、0.15%、0.20%的富锌益生菌,试验期55 d,试验结束时分离血清,测定血清中总超氧化物歧化酶(T-SOD)活性、总抗氧化能力(T-AOC活性)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量;选取500只1日龄健康海兰褐雏鸡,分为空白对照组(饲喂基础日粮)、3个富锌益生菌组(分别在基础日粮中添加0.10%、0.15%、0.20%的富锌益生菌)、抗生素对照组(在基础日粮中添加0.15%的金霉素)、攻毒对照组,除空白对照组外,其他组均分别下设大肠杆菌攻毒组、鸡白痢沙门氏菌攻毒组、鸡葡萄球菌攻毒组,试验期49 d,研究富锌益生菌对大肠杆菌、鸡白痢沙门氏菌、鸡葡萄球菌的抗感染能力。结果表明,与对照组相比,0.15%、0.20%富锌益生菌组TSOD活性分别显著提高了10.16%、13.01%,MDA含量分别显著降低了17.99%、30.67%;0.10%、0.15%、0.20%富锌益生菌组T-AOC活性分别显著提高14.54%、22.85%、27.01%,GSH-Px活性提高,但差异均不显著。攻毒试验结果表明,0.10%、0.15%、0.20%富锌益生菌组大肠杆菌发病率分别降低了20.00%、46.67%、53.33%,死亡率分别降低了30.44%、65.21%、73.91%;鸡白痢沙门氏菌发病率分别降低了32.14%、42.86%、53.57%,死亡率分别降低了31.58%、52.63%、73.68%;鸡葡萄球菌发病率分别降低了36.00%、48.00%、55.99%,死亡率分别降低了55.56%、66.67%、77.77%。综上,富锌益生菌能够提高蛋鸡的抗氧化机能和抵抗病原微生物感染的能力。
[Abstract]:In order to study the effect of zinc-rich probiotics prepared by beneficial bacteria into inorganic zinc in the production of laying hens, 120 Hailan brown laying hens aged 19 weeks were divided into control group and three experimental groups. The control group was fed with basal diet.The experimental group added 0.10% 0.15% zinc rich probiotics to the basal diet, 55 days after the experiment, the serum was isolated at the end of the experiment, and the total superoxide dismutase (T-SOD) activity in the serum was determined.The activity of T-AOC, glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the total antioxidant capacity of 500 1-day-old Hailan brown chicks were selected.They were divided into blank control group (fed basal diet), three zinc-rich probiotics groups (0.10% 0.15% Zinc-rich probiotics 0.20% were added to basal diet, antibiotic control group (0.15% chlortetracycline added to basal diet, attack control group, except blank control group).Other groups were divided into Escherichia coli attack group, chicken bacillus pullorum attack group, chicken staphylococcus attack group for 49 days, to study the anti-infection ability of Zinc-rich probiotics against Escherichia coli, Chicken Salmonella pullorum and Staphylococcus Chicken.The results showed that compared with the control group, the activity of TSOD in 0.20% Zinc-enriched probiotics group increased significantly, but the T-AOC activity in the Zinc-enriched probiotics group increased significantly (14.5422.8527.01%), but there was no significant difference in the activity of GSH-Px between the two groups.The results showed that the incidence of Escherichia coli in 0.10% 0.20% Zinc-rich probiotics group was reduced by 20.00 ~ 46.67% and the mortality rate was reduced by 30.4444 ~ 65.21% and 73.91% respectively, and the incidence of Salmonella pullorum was reduced by 32.1442.86% ~ 53.57%, and the mortality was reduced by 31.58% and 52.63%, respectively, and the incidence of Staphylococcus pullorum was decreased by 31.58% and 52.63%, respectively.36.00 and 48.00, respectively, and the death rate was reduced by 55.56 and by 66.67 and 77.77, respectively.In conclusion, Zinc-rich probiotics can improve the antioxidant function and resistance to pathogenic microorganism infection of laying hens.
【作者单位】： 吉林大学动物医学学院;河南牧业经济学院动物医学院;
【基金】：河南省科技攻关项目(132102313102)
【分类号】：S831.5
，

本文编号：1761952