建立柱前衍生化-HPLC法测定发酵液中L-蛋氨酸

发布时间：2018-04-17 05:11

  本文选题：蛋氨酸 + 柱前衍生化　； 参考：《畜牧与兽医》2017年01期

【摘要】：拟建立一种简单、快速、高效的柱前衍生化HPLC法测定发酵液中蛋氨酸含量。以2,4-二硝基氯苯(CDNB)为衍生化试剂,采用高效液相色谱法,定量测定发酵液中的蛋氨酸含量。色谱条件:流动相0.01 mol/L p H 5.2 Na AC-乙腈(70∶30);检测波长:350 nm;柱温:25℃;流速:0.8 m L/min。结果表明:蛋氨酸含量在10~80μg/m L范围内峰面积与浓度呈现良好的线性关系,R2=0.999 24;检测限和定量限分别为0.1和0.5μg/m;加样回收率为98.7%,并且具有良好的重复性。
[Abstract]:To establish a simple, rapid and efficient HPLC method for the determination of methionine in fermentation broth.The methionine content in fermentation broth was quantitatively determined by HPLC with 2 '4-dinitrochlorobenzene (CDNB) as derivatization reagent.The chromatographic conditions were as follows: mobile phase 0.01 mol/L pH 5.2 Na AC- acetonitrile 70: 30; detection wavelength: 350nm; column temperature: 25 鈩，

本文编号：1762205