茶皂素对奶牛瘤胃原虫区系的影响

发布时间：2018-04-17 08:46

本文选题：奶牛 + 原虫区系　；参考：《动物营养学报》2017年02期

【摘要】：本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛[体重(550±30)kg,产奶量35 kg/(头·d),胎次2~4],随机分为4组,每组3头,均饲喂基础饲粮,分别于晨饲前灌服0(对照)、20、30、40 g/(头·d)的茶皂素。试验期49 d,其中预试期14 d,正试期35 d,正试期内每7 d于晨饲前1 h用口腔采样器采集瘤胃液。采用聚丙烯酰氨凝胶电泳(DGGE)结合18S r DNA序列分析技术研究瘤胃原虫区系的变化。结果表明:1)添加茶皂素可以选择性地抑制瘤胃中原虫的生长。2)与对照组相比,30、40 g/(头·d)茶皂素组的丰富度指数和香农多样性指数都显著降低(P0.05),优势度指数显著增加(P0.05),但是均一性指数无显著变化(P0.05);茶皂素显著减少了瘤胃中前庭亚纲内毛目的原虫数量。综上所述,饲粮中添加30、40 g/(头·d)茶皂素均可抑制奶牛瘤胃原虫的生长,并且降低瘤胃原虫的多样性。
[Abstract]:The purpose of this study was to study the effect of tea saponin on rumen protozoa of dairy cows.Twelve healthy Holstein cows [body weight 550 卤30 kg, milk yield 35 kg / (head dome, second parity 4)] were randomly divided into 4 groups, each group (3 cows) were fed with basic diet and were fed with tea saponin of 40 g / r (head d) before morning feeding.The test period was 49 days, in which the pre-test period was 14 days, the positive test period was 35 days, and the oral sampler was used to collect rumen fluid 1 hour before morning feeding every 7 days during the trial period.The floristic changes of rumen protozoa were studied by polyacrylamide gel electrophoresis and 18s r DNA sequence analysis.The results showed that: 1) addition of tea saponin could selectively inhibit the growth of protozoa in rumen. 2) compared with the control group, the richness index and Shannon diversity index of 3040 g / (head d) tea saponin group decreased significantly (P 0.05), and the dominance index increased significantly.P0.05, but the homogeneity index was not significantly changed. Tea saponin significantly reduced the number of Trichozoidae protozoa in the vestibule subclass in the rumen.In conclusion, 30% 40 g / (head d) tea saponin in diet could inhibit the growth of rumen protozoa and reduce the diversity of rumen protozoa.
【作者单位】：北京农学院动物科学技术学院奶牛营养学北京市重点实验室;中国农业科学院畜牧研究所;
【基金】：“十二五”国家科技支撑项目(25012BAD14B09) “十三五”国家重大科技专项(2016YFDO70020,2016YFDO700205) 北京市农业局北京市现代农业产业技术体系奶牛创新团队
【分类号】：S823.5

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