黄芪灵芝口服液促进雏鸡免疫功能的研究

发布时间：2018-04-17 10:22

本文选题：黄芪 + 灵芝　；参考：《扬州大学》2015年硕士论文

【摘要】：黄芪和灵芝是传统的中草药,主要活性成分为黄芪多糖和灵芝多糖,具有免疫增强作用。黄芪灵芝口服液为某生物技术公司研制的新产品,黄芪多糖和灵芝多糖为其有效成分。本文研究黄芪灵芝口服液对正常及免疫抑制模型雏鸡免疫功能及ND疫苗免疫作用的影响,旨在探讨黄芪灵芝口服液的免疫增强作用,为黄芪灵芝口服液作为一种新型免疫增强剂应用于兽医临床提供实验依据。对雏鸡免疫功能的影响研究：7日龄黄羽肉鸡180只,随机分成9组。第1-3组分别按10、5、1 mL/kg剂量灌服黄芪灵芝口服液,第4-7组分别按80 mg/kg剂量肌内注射环磷酰胺复制免疫抑制模型,第4-6组同时分别给予10、5、1 mL/kg黄芪灵芝口服液,第8-9组分别按1 mL/kg剂量灌服阳性对照物黄芪精口服液和生理盐水,黄芪灵芝口服液、黄芪精口服液及生理盐水连用5天,环磷酰胺连用3天。分别于12、17、22日龄从各组随机采样,测定免疫器官指数、脾淋巴细胞增殖及血清中IL-2水平。结果表明,黄芪灵芝口服液高、中、低剂量组在12、17、22日龄时,脾淋巴细胞增殖显著高于生理盐水对照组和免疫抑制模型组(P0.05)。22日龄时,黄芪灵芝口服液高、中、低剂量组IL-2水平显著高于生理盐水对照组和免疫抑制模型组(P0.05)。黄芪灵芝口服液中剂量组的胸腺和法氏囊指数显著高于生理盐水对照组和免疫抑制模型组(P0.05)。黄芪灵芝口服液灌服能促进雏鸡免疫功能,在一定程度上能有效拮抗由环磷酰胺引起的免疫抑制。对雏鸡ND疫苗免疫作用的影响研究：14日龄黄羽肉鸡180只,随机分成9组。1-9组通过滴眼滴鼻接种新城疫活疫苗,第1～3组分别按10、5、1 mL/kg剂量灌服黄芪灵芝口服液,第4～7组分别按80 mg/kg剂量肌内注射环磷酰胺,第4-6组同时分别给予按10、5、1 mL/kg黄芪灵芝口服液,第8组以1 mL/kg剂量灌服黄芪精口服液阳性对照物；第9组给予生理盐水作为疫苗对照组。黄芪灵芝口服液、黄芪精口服液及生理盐水连用5天,环磷酰胺连用3天。分别于免疫后第7、14、21、28天从各组随机采样,测定免疫器官指数、脾淋巴细胞增殖、血清中IL-2水平及ND抗体滴度。结果表明,黄芪灵芝口服液高、中、低剂量组脾淋巴细胞增殖在免疫后第7、14、21、28天显著高于疫苗对照组(P0.05)；IL-2水平在免疫后3w显著高于疫苗对照组(P0.05)；免疫后1 w,受试物所有剂量组的抗体滴度均高于疫苗对照组,且抗体滴度维持的时间与疫苗组相当,黄芪灵芝口服液能促进ND疫苗免疫雏鸡后的抗体产生。综上所述,黄芪灵芝口服液可增强雏鸡免疫功能及ND疫苗的免疫作用,可望作为免疫增强剂使用。
[Abstract]:Astragalus membranaceus and Ganoderma lucidum are traditional Chinese herbal medicines. The main active components are astragalus polysaccharide and Ganoderma lucidum polysaccharide.Astragalus ganoderma oral liquid is a new product developed by a biotechnology company. Astragalus polysaccharides and Ganoderma lucidum polysaccharides are effective components.The effects of Huangqi Ganoderma lucidum oral liquid on immune function and ND vaccine immunity of normal and immunosuppressive chicks were studied in order to explore the immune enhancement effect of Huangqi Ganoderma lucidum oral liquid.To provide experimental evidence for the application of Astragalus Ganoderma oral liquid as a new immune enhancer in veterinary clinic.Study on immune function of Chicks 180 yellow broilers aged 7 days were randomly divided into 9 groups.Group 1-3 was given Astragalus Ganoderma oral liquid at a dose of 10 mL/kg, group 4-7 was injected intramuscularly with cyclophosphamide at a dose of 80 mg/kg to establish immunosuppressive model, group 4-6 was given 10 mL/kg Astragalus ganoderma oral liquid at the same time, and group 4-7 was injected intramuscularly with cyclophosphamide at a dose of 80 mg/kg.In group 8-9, Huangqi essence oral liquid and normal saline, astragalus lucidum oral liquid, astragalus essence oral liquid and normal saline were given at the dose of 1 mL/kg for 5 days, and cyclophosphamide for 3 days.The immune organ index, splenic lymphocyte proliferation and serum IL-2 level were measured by random sampling at the age of 1722 days.The results showed that the proliferation of splenic lymphocytes in Astragalus lucidum oral liquid was significantly higher than that in normal saline control group and immunosuppressive model group at the age of 12 days 1722 days, and the oral liquid of Astragalus lucidum was high and medium at 22 days of age, and the dose of Astragalus Ganoderma lucidum oral liquid was higher than that of normal saline control group and immunosuppressive model group.The level of IL-2 in low dose group was significantly higher than that in normal saline control group and immunosuppressive model group (P 0.05).The thymus and bursa index of middle dose group of Huangqi Ganoderma lucidum oral liquid was significantly higher than that of normal saline control group and immunosuppressive model group (P 0.05).Huangqi Ganoderma lucidum oral solution could promote the immune function of chicks and antagonize the immunosuppression caused by cyclophosphamide to some extent.Effects of ND Vaccine on immunity of Chicks 180 broilers aged 14 days were randomly divided into 9 groups. Group 1-9 was given live Newcastle disease vaccine by intranasal drip. The first and third groups were given Astragalus Ganoderma oral solution at the dose of 10 ~ 51g / mL/kg, respectively.In group 4, cyclophosphamide was injected intramuscularly at the dose of 80 mg/kg, group 4-6 was given Astragalus ganoderma oral liquid for 10 mL/kg, and group 8 was administered with 1 mL/kg dose of Astragalus essence oral liquid as positive control.Group 9 was given normal saline as vaccine control group.Astragalus lucidum oral liquid, astragalus essence oral liquid and normal saline were used for 5 days and cyclophosphamide for 3 days.The immune organ index, spleen lymphocyte proliferation, serum IL-2 level and ND antibody titer were measured by random sampling on day 28 after immunization.The results showed that Huangqi Ganoderma lucidum oral liquid was high and moderate.The proliferation of spleen lymphocytes in the low dose group was significantly higher than that in the vaccine control group on the 28th day after immunization, and the level of IL-2 was significantly higher than that in the vaccine control group at 3 weeks after immunization, and the titer of antibody in all dose groups was higher than that in the vaccine control group at 1 week after immunization.Astragalus lucidum oral liquid could promote antibody production after ND vaccine immunization.To sum up, Astragalus Ganoderma oral liquid can enhance the immune function of chicks and the immune effect of ND vaccine, which is expected to be used as immune enhancer.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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