福建猫源弓形虫IgG抗体检测及PCR-RFLP基因分型与遗传变异分析

发布时间：2018-04-17 17:41

本文选题：猫 + 弓形虫　；参考：《中国兽医科学》2017年12期

【摘要】：采用酶联免疫吸附(ELISA)试验对福建某花鸟市场采集的40份猫血液样本进行弓形虫IgG抗体检测,对抗体呈现阳性的血液样品进行弓形虫B1基因PCR扩增;对扩增结果呈现阳性的样本采用多位点巢式PCR-RFLP技术,在SAG1、5’SAG2、3’SAG2、Alt.SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Api co共12个位点进行弓形虫基因分型研究和部分位点的遗传变异分析。结果表明,40份血清中,19份血清呈弓形虫IgG抗体阳性,阳性率为47.5%,19份血清抗体呈阳性的血液样品中,B1基因PCR扩增出3份阳性样品,进一步多位点巢式PCR-RFLP分析和遗传变异分析发现,PCR阳性样品中的弓形虫基因型均表现为New Genotype型。
[Abstract]:Toxoplasma gondii (Toxoplasma gondii) IgG antibody was detected by enzyme linked immunosorbent assay (Elisa) in 40 cat blood samples collected from a flower and bird market in Fujian Province. Toxoplasma gondii B1 gene was amplified by PCR in the blood samples which were positive for Toxoplasma gondii.Multilocus nested PCR-RFLP technique was used to study the genotyping and genetic variation of Toxoplasma gondii at 12 loci of SAG1, SAG2, Alt.SAG2, SAG3, BTUBMA6C22-8C29-2L358PK1 and Api co., which were positive for Toxoplasma gondii (Toxoplasma gondii). The results showed that there were 12 loci of Toxoplasma gondii (Toxoplasma gondii).The results showed that 19 out of 40 sera were positive for Toxoplasma gondii IgG antibody, and the positive rate was 47.5%. The positive rate was 47.5%. Three positive samples were amplified by PCR of B 1 gene in 19 blood samples with positive serum antibody to Toxoplasma gondii (Toxoplasma gondii).Further nested PCR-RFLP analysis and genetic variation analysis showed that Toxoplasma gondii genotypes were New Genotype type in the positive samples of Toxoplasma gondii (Toxoplasma gondii).
【作者单位】：龙岩学院生命科学学院;福建省家畜传染病防治与生物技术重点实验室;福建农林大学动物科学学院福建省动物药物工程实验室;
【基金】：福建省科技厅省属高校科研项目(Jk2011052) 龙岩市科技计划项目(2012LY45)
【分类号】：S852.7

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