全蛋白组水平预测旋毛虫分泌蛋白及其功能分析

发布时间：2018-04-18 02:14

本文选题：旋毛虫 + 蛋白组　；参考：《畜牧与兽医》2017年04期

【摘要】：从蛋白组水平预测并分析旋毛虫分泌蛋白的特征。利用软件预测旋毛虫蛋白组范围分泌蛋白,并统计和分析其序列特征,最后利用数据库信息注释分析分泌蛋白功能。结果:预测得到414个经典途径分泌蛋白和245个非经典途径分泌蛋白,占蛋白组2.40%。MEME统计经典分泌蛋白信号肽中存在可能的3个基序。氨基酸组成分析表明,旋毛虫分泌蛋白及其信号肽主要由疏水性氨基酸组成,而信号肽剪切位点主要由亲水性氨基酸组成,信号肽剪切位点-3和-1两个位点氨基酸保守性高,可能是相关酶识别关键点。在对分泌蛋白的功能进行注释分析中发现,与核酸酶活性和蛋白酶活性相关的蛋白比例较高。研究结果为旋毛虫致病机制的深入研究提供理论基础。
[Abstract]:The characteristics of Trichinella spiralis secretory protein were predicted and analyzed from the proteome level.The software was used to predict the secretory proteins of Trichinella spiralis, and the sequence characteristics of them were analyzed. Finally, the function of secreting proteins was analyzed by annotating the database information.Results: a total of 414 classical pathway secretory proteins and 245 non-classical pathway secreted proteins were predicted, accounting for three possible motifs in the 2.40%.MEME of the protein group.Amino acid composition analysis showed that the secreted protein and its signal peptide of Trichinella spiralis were mainly composed of hydrophobic amino acids, while the signal peptide shearing sites were mainly composed of hydrophilic amino acids, and the amino acids of signal peptide shearing sites-3 and -1 were highly conserved.It may be the key point of enzyme recognition.In the analysis of the function of secretory protein, it was found that the proportion of protein related to nuclease activity and protease activity was high.The results provide a theoretical basis for the further study of the pathogenic mechanism of Trichinella spiralis.
【作者单位】：南京农业大学动物医学院;
【基金】：江苏省自然科学基金(BK20141365)
【分类号】：S852.7

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