沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响

发布时间：2018-04-18 07:28

本文选题：沙葱黄酮 + 肉羊　；参考：《动物营养学报》2017年03期

【摘要】：本试验旨在研究饲粮中添加沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响。选用60只6月龄左右、体重(39.9±3.2)kg的小尾寒羊为试验动物,按照出生月龄和体重相近的原则,随机分为4组,每组15只羊。对照组饲喂基础饲粮,3个试验组分别在基础饲粮中添加11、22、33 mg/kg的沙葱总黄酮。预试期为15 d,正试期为60 d。正试期第60天空腹颈静脉采血,采用噻唑蓝(MTT)法测定肉羊外周血淋巴细胞转化率,采用流式细胞仪测定细胞周期、细胞凋亡率。结果表明:1)饲粮添加11、22、33 mg/kg沙葱黄酮可提高肉羊外周血淋巴细胞转化率,其中33 mg/kg组显著高于对照组(P0.05);2)与对照组相比,饲粮添加33 mg/kg沙葱黄酮能够显著提高S期和G2/M期细胞比例(P0.05);3)与对照组和11 mg/kg组相比,22和33 mg/kg组外周血淋巴细胞凋亡率显著提高(P0.05)。由此可见,饲粮中添加11~33 mg/kg沙葱黄酮可促进肉羊外周血淋巴细胞转化,并使细胞向DNA合成期转化,促进细胞分裂,对细胞凋亡有显著促进作用,以添加33 mg/kg沙葱黄酮的效果最佳。
[Abstract]:The purpose of this study was to study the effects of flavanone on lymphocyte transformation and apoptosis in peripheral blood of meat sheep.60 small tail Han sheep of about 6 months old and 39.9 卤3.2)kg weight were selected as experimental animals. According to the principle of similar birth age and body weight, 60 small tail Han sheep were randomly divided into 4 groups with 15 sheep in each group.The control group was fed with basic diet, and the three experimental groups were fed with total flavonoids of Allium mongolicum for 11 ~ 22 ~ 33 mg/kg respectively.The pre-test period was 15 days and the positive test period was 60 days.Fasting jugular vein blood was collected on the 60th day of the trial period. The lymphocyte transformation rate in peripheral blood of sheep was measured by thiazolyl methoate (MTT) method. The cell cycle and apoptosis rate were measured by flow cytometry (FCM).The results showed that the lymphocyte transformation rate in peripheral blood of meat sheep was significantly higher in 33 mg/kg group than that in control group (P 0.05).Compared with control group and control group and 11 mg/kg group, the apoptosis rate of peripheral blood lymphocytes in the diet supplemented with 33 mg/kg flavanone significantly increased the ratio of cells in S phase and G 2 / M phase (P 0.05N 3). The apoptosis rate of peripheral blood lymphocytes in the control group and 33 mg/kg group was significantly higher than that in the control group and 11 mg/kg group.It can be seen that the addition of 1133 mg/kg flavanone in diet can promote the transformation of peripheral blood lymphocytes and the transformation of cells to DNA synthesis, promote cell division, and promote apoptosis.The best effect was obtained by adding 33 mg/kg flavonoids from Allium mongolicum.
【作者单位】：内蒙古农业大学动物科学学院;河套学院农学系;
【基金】：国家自然科学基金地区科学基金项目(31260558,31160474) “十二五”国家科技支撑计划(2013BDA10B04)
【分类号】：S826.5

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