复合抗菌肽“态康利保”对断奶仔猪体液免疫及细胞因子的影响

发布时间：2018-04-18 12:33

  本文选题：复合抗菌肽“态康利保” + 仔猪　； 参考：《四川农业大学》2015年硕士论文

【摘要】：试验研究了不同剂量的复合抗菌肽制剂“态康利保”对断奶仔猪体液免疫及细胞因子水平的影响,并探索“态康利保”的最佳添加量及其作用机理,力求找到一种绿色、高效的天然免疫增强剂。试验选取17日龄接种猪瘟兔化弱毒疫苗、21日龄断奶的临床健康且猪瘟抗体水平相近(25日龄检测猪瘟抗体水平)的仔猪90头,随机分为5组。试验期为26-53日龄,Ⅰ组饲喂基础日粮,Ⅱ组在饲料中添加黄芪多糖400mg/kg, Ⅲ、 Ⅳ、Ⅴ组在饲料中分别添加“态康利保'250mg/kg、500mg/kg、1000mg/kg。分别在32日龄、39日龄、46日龄、53日龄每组随机选取6头仔猪前腔静脉采血,分离血清后分别用于猪瘟抗体水平、免疫球蛋白(IgG、IgM、IgA)、总补体(ch50)及细胞因子(IL-2、IL-4、IL-6、IFN-γ、TNF-α)含量的测定。试验结果：(1)“态康利保”和黄芪多糖均能提高猪瘟抗体水平,且“态康利保”维持抗体水平更为持久,以“态康利保”1000mg/kg添加量效果最为明显。(2)“态康利保'1000mg/kg饲料组可明显提高仔猪血清中IgG、IgA及IgM的含量(P0.05或P0.01),且以连续饲喂3周效果最为明显。“态康利保”500mg/kg饲料组与黄芪多糖组均可提高血清IgG含量,但两组差异不明显；“态康利保"500mg/kg饲料组可有效提高血清IgM水平；“态康利保’'1000mg/kg组能较早提高血清Iga含量。 (3)“态康利保”和黄芪多糖均能极显著的提高血清ch50含量(P0.01),且“态康利保”组优于黄芪多糖组,以“态康利保”1000mg/kg添加量最为明显。 (4)“态康利保”可提高血清中Th 1型IL-2、IFN-γ、TNF-α及Th2型IL-4、IL-6含量。“态康利保”1000mg/kg组与对照组相比,可极显著提高血清IL-2、IL-6、IFN-γ和TNF-α含量(P0.01),从39日龄起,“态康利保”1000mg/kg饲料组可显著提高血清IL-4水平(P0.01)；黄芪多糖可一定程度提高血清IL-2、IL-4、IL-6、IFN-γ和TNF-α的含量,但效果不及“态康利保”。结论：复合抗菌肽“态康利保”可明显提高断奶仔猪猪瘟抗体水平、免疫球蛋白、总补体ch50、细胞因子的含量,从而提高仔猪的免疫力。以日粮中添加1000mg/kg剂量连续饲喂3周以上效果最佳。
[Abstract]:The effects of different doses of compound antimicrobial peptide preparation "State Kanglibao" on humoral immunity and cytokine levels in weaned piglets were studied, and the optimum dosage and mechanism of "state Kanglibao" were explored to find a green.A highly effective innate immune enhancer.A total of 90 piglets were randomly divided into 5 groups, which were randomly divided into 5 groups, which were randomly divided into 5 groups, which were randomly divided into 5 groups, and 90 piglets inoculated with the attenuated hog fever vaccine at the age of 17 days and weaned at 21 days of age with similar levels of antibody to swine fever were randomly divided into 5 groups.The trial period was 26 to 53 days old. Group I was fed basic diet, group 鈪，

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