H5N1亚型禽流感灭活疫苗和DNA疫苗诱导IFN-γ应答评价

发布时间：2018-04-18 15:44

本文选题：HN亚型禽流感病毒 + 灭活疫苗　；参考：《扬州大学学报(农业与生命科学版)》2017年03期

【摘要】：用H5N1亚型禽流感病毒(AIV)油乳剂灭活疫苗和携带H5N1亚型AIV血凝素(HA)的重组DNA疫苗pVAX1-HA分别免疫2周龄SPF鸡,共免疫3次,每次间隔4周。每次免疫后14d,每组随机取4只鸡,分离其脾脏淋巴细胞,用H5N1亚型AIV JS株灭活全病毒作为特异性体外刺激抗原刺激鸡脾脏淋巴细胞,72h后,检测其培养上清中特异性ChIFN-γ的水平,同时用HI试验检测免疫鸡血清抗体。结果表明:重组DNA疫苗pVAX1-HA免疫组在二次免疫后,可检测出特异性ChIFN-γ的分泌和一定水平的抗体应答;而H5N1亚型AIV灭活疫苗免疫组均未能检测出ChIFN-γ,二次免疫后能产生较高水平的抗体应答。说明DNA疫苗可激发较高水平的IFN-γ应答,而AIV灭活疫苗主要激发抗体应答,提示两者联合免疫可使免疫的禽群诱导产生强的细胞免疫应答和抗体应答,为AIV的防控提供了参考。
[Abstract]:Two week-old SPF chickens were immunized with H5N1 subtype AIV oil emulsion inactivated vaccine and recombinant DNA vaccine pVAX1-HA carrying H5N1 subtype AIV hemagglutinin.On the 14th day after each immunization, four chickens were randomly selected from each group and their spleen lymphocytes were isolated. After 72 hours of stimulation with H5N1 subtype AIV JS strain as specific stimulation antigen in vitro, the level of specific ChIFN- 纬 in culture supernatant was detected.At the same time, HI test was used to detect antibody in serum of immunized chicken.The results showed that the specific secretion of ChIFN- 纬 and antibody response could be detected in the recombinant DNA vaccine pVAX1-HA immunized group after secondary immunization.However, ChIFN- 纬 could not be detected in the H5N1 subtype AIV inactivated vaccine immunization group, and a higher level of antibody response could be produced after secondary immunization.The results indicated that the DNA vaccine could induce a higher level of IFN- 纬 response, while the inactivated AIV vaccine mainly stimulated antibody response, suggesting that combined immunization could induce strong cellular immune response and antibody response in the vaccinated poultry, which provided a reference for the prevention and control of AIV.
【作者单位】：扬州大学江苏省人兽共患病学重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心/医学院;
【基金】：国家自然科学基金资助项目(30972171、81472815) 江苏省优势学科建设工程项目(2014-05)
【分类号】：S852.4

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