母源抗体对感染PCV2仔猪体内病毒变化和免疫反应的影响

发布时间：2018-04-19 04:01

本文选题：猪圆环病毒Ⅱ型 + 断奶仔猪多系统衰竭综合征　；参考：《中国兽医学报》2017年03期

【摘要】：将16头断奶仔猪随机分成两大组即直接感染组和间接感染组,再将每大组用ELISA测抗体水平并排序分成两小组,即高抗体水平的4头为高起始抗体组,低抗体4头为低起始抗体组。直接感染组注射PCV2攻毒后再注射钥匙孔血蓝蛋白(KLH)刺激,间接感染组不注射与直接感染组混养。Q-PCR检测血清中病毒拷贝数,血常规检测全血中淋巴细胞数,定时称量体质量,屠宰后采样制作HE染色病理切片。结果,HE染色病理切片和临床分析显示感染猪只表现断奶仔猪多系统衰竭综合征(PMWS)症状;直接感染组和间接感染组攻毒后抗体水平持续降低,且分别在攻毒后20、30d降到相对最低值。直接感染组在攻毒后4d检测到抗原,攻毒后7d低起始抗体组病毒拷贝数显著高于高起始抗体组,间接感染组分别在攻毒后4、7d检测出抗原,攻毒后24、29d低起始抗体组抗原显著高于高起始抗体组;两组中外周血淋巴细胞数量在攻毒后减少,攻毒期间两大组中低起始抗体组和高起始抗体组体质量无差异。结果表明,PCV2+KLH能够复制出PMWS症状;PCV2母源抗体的半衰期为15d左右;低起始抗体组更容易感染病毒;攻毒后起始母源抗体的高低对断奶仔猪增重差异不明显。
[Abstract]:Sixteen weaned piglets were randomly divided into two groups: direct infection group and indirect infection group. The antibody levels of each group were measured by ELISA and sequenced into two groups.Four of the low antibodies were in the low initial antibody group.The direct infection group was injected with PCV2 and then the keyhole hemocyanin (KLH) stimulation was injected. The indirect infection group was not injected with direct infection group. Q-PCR was used to detect the virus copy number in serum, blood routine examination of lymphocyte number in the whole blood, timing weighing body weight.After slaughter, samples were taken to make HE stained pathological sections.Results pathological sections and clinical analysis of HE staining showed that infected piglets showed the symptoms of multiple system failure syndrome (PMWS), and the antibody level of direct infection group and indirect infection group decreased continuously after attack, and decreased to the lowest level at 2030 days after inoculation.The antigens were detected in the direct infection group on the 4th day after the attack. The copy number of the virus in the low initial antibody group was significantly higher than that in the high initial antibody group on the 7th day after the attack, and the antigen was detected in the indirect infection group at 4 and 7 days after the attack.The number of peripheral blood lymphocytes in the low initial antibody group was significantly higher than that in the high initial antibody group, but there was no difference in body mass between the low and low initial antibody groups and the high initial antibody group during the attack.The results showed that the half-life of mother antibody of PCV2 KLH was about 15 days, and that of low initial antibody group was more likely to infect virus, and there was no significant difference in weight gain of weaning piglets.
【作者单位】：华南农业大学动物科学学院国家生猪种业工程技术研究中心;
【基金】：广东省现代农业产业技术体系生猪创新团队资助项目(2016LM1101,2016LM1104)
【分类号】：S858.28

【参考文献】