温热病初期治疗作用方剂莲蓝口服液的研制

发布时间：2018-04-19 07:51

本文选题：莲蓝口服液 + 制备工艺　；参考：《河南科技大学》2015年硕士论文

【摘要】：目的:治疗温热病初期的中药方剂较多,其中中国兽药典中收载的有“清解合剂”、“清温败毒散”、“银翘散”、“双黄连口服液”等,这些方剂的药材价格较昂贵、生产成本高,限制其在养殖生产中的使用。为选择高效价廉的预防治疗方剂,本试验选用板蓝根、大青叶、穿心莲三味中药重新组方并对其提取工艺、药效学、质量控制、毒理学进行研究,旨在研制对温热病初期的防治具有确切效果、性价比高的中兽药方剂。方法:(1)以R-S告依春、穿心莲内酯和脱水穿心莲内酯的含量测定为依据,筛选各成分含量均较高的莲蓝口服液提取方法及其组方。(2)用制备实验中的三种口服液样品对温热病初期小白鼠模型进行预防及治疗的药效学实验,以确定莲蓝口服液的最佳组方。(3)通过对穿心莲、板蓝根、大青叶进行薄层鉴别,穿心莲和板蓝根的含量测定等方法对筛选出的方剂进行质量可控性研究。(4)对小鼠进行急性毒性实验,以及对大鼠进行连续给药30天的长期毒性试验,来进行莲蓝口服液的安全性评价。结果:(1)莲蓝口服液的制备实验中,板蓝根和大青叶提取液用水提醇沉法获得,穿心莲用75%乙醇温浸提取,板蓝根、大青叶与穿心莲提取液按原药材2:1:1的比例混合后制得的莲蓝口服液各标示成分含量较高。(2)药效学实验中,板蓝根、大青叶与穿心莲提取液按原药材2:1:1的比例混合后制得的莲蓝口服液的解热效果最好。(3)分别建立了莲蓝口服液中大青叶、板蓝根和穿心莲的薄层鉴别方法和大青叶、穿心莲的含量测定方法。质量研究表明,方法均无阴性干扰,可以用来控制莲蓝口服液的质量。(4)急性毒性试验中小鼠给药量达40000 mg/kg体重,7天内所有小鼠没有死亡,各组剖解检查亦无明显改变;长期毒性试验中各给药组动物的体重增重、血常规、血液生化学指标与对照组相比均无显著性差异,各组剖检及组织病理学检查亦无明显改变。说明莲蓝口服液没有明显的毒性作用。结论:莲蓝口服液的制备工艺简单快速,药效显著,建立的检测方法可行、稳定,可有效地控制莲蓝口服液的质量。此组方制备的莲蓝口服液未见明显毒性,可进一步开发为新兽药。
[Abstract]:Objective: there are many traditional Chinese medicine prescriptions for the treatment of warm fever. Among them, the Chinese veterinary pharmacopoeia contains "Qingjie mixture", "Qingwen Baidu Powder", "Yinqiao Powder", "Shuanghuanglian Oral liquid" and so on.High production cost limits its use in aquaculture production.In order to select effective and inexpensive preventive and therapeutic prescriptions, we selected Radix Isatidis, Radix Isatidis and andrographis andrographis, and studied their extraction technology, pharmacodynamics, quality control and toxicology.The purpose of this study was to develop a Chinese veterinary medicine prescription with high performance and cost-effective in the early stage of prevention and treatment of warm fever.Methods the contents of andrographolide and dehydrated andrographolide were determined on the basis of R-S Gouyichun.To select the extraction method of Lianlan oral liquid with high content of each component and its formula. 2) to use the three kinds of oral liquid samples in the preparation experiment to carry on the pharmacodynamics experiment to prevent and treat the mice model of febrile fever in the early stage.In order to determine the best prescription of Lianlan oral liquid, through thin layer identification of andrographis andrographis, Radix Isatidis and Radix Isatidis,The contents of andrographis andrographis and Radix Isatidis were determined by the methods of quality controllability of the selected prescription. The acute toxicity test was carried out in mice and the long-term toxicity test was carried out in rats for 30 days.To evaluate the safety of Lianlan oral liquid.Results in the preparation experiment of Lianlan oral liquid, Isatis Isatidis and Radix Isatidis were obtained by water extraction and alcohol precipitation, andrographis andrographis was extracted by 75% ethanol, and Radix Isatidis was extracted.Radix Isatidis and andrographis andrographis extract were mixed at 2:1:1 of the original medicinal materials, and the contents of each marked components of Lianlan oral liquid were higher than that of Radix andrographis) in the pharmacodynamics experiment, Isatidis root, Radix Isatidis, Radix Isatidis,The best antipyretic effect of Lianlan oral liquid was obtained by mixing the extract of Daqingye and andrographis andrographis according to the proportion of the original medicine at 2:1:1). The thin layer identification method of Daqingye, Isatidis root and andrographis andrographis in Lianlan oral liquid was established respectively.Determination of andrographis paniculata content.The quality study showed that all the methods had no negative interference and could be used to control the quality of Lianlan oral liquid.There was no significant difference in weight gain blood routine blood biochemical indexes and histopathological examination between each group and the control group in long-term toxicity test.It indicated that Lianlan oral liquid had no obvious toxic effect.Conclusion: the preparation process of Lianlan oral liquid is simple and rapid, and its pharmacodynamics is remarkable. The established method is feasible and stable, and can effectively control the quality of Lian Lan oral liquid.Lianlan oral liquid prepared by this prescription has no obvious toxicity and can be further developed as a new veterinary drug.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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