香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响

发布时间：2018-04-19 09:13

本文选题：香菇多糖 + 大肠杆菌　；参考：《动物营养学报》2017年10期

【摘要】：本研究旨在探讨香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响。将24只SD大鼠随机分成4个组(A、B、C、D组),每组6个重复,每个重复1只。A、B组饮用蒸馏水,C、D组饮用蒸馏水中添加20μg/m L的香菇多糖;试验第15天B、D组灌服2 m L浓度为1×1010CFU/m L大肠杆菌K88,A、C组灌服等量生理盐水。试验第18天心脏采血处死,取空肠固定和冻存,制作石蜡切片,苏木精-伊红(HE)染色和高碘酸雪夫氏(PAS)染色,测定绒毛高度、隐窝深度、绒毛宽度、上皮内淋巴细胞(IEL)数量和上皮杯状细胞(GC)数量,计算绒毛高度与隐窝深度的比值(V/C);并采用蛋白质印迹(Western-blot)法测定空肠紧密连接蛋白Occludin的表达水平。结果表明:各组绒毛高度和绒毛宽度无显著差异(P0.05)。C组隐窝深度极显著低于A、B组(P0.01),显著低于D组(P0.05)。C组V/C极显著高于A、B、D组(P0.01)。C组IEL数量极显著低于B、D组(P0.01),显著低于A组(P0.05)。C组上皮GC数量极显著高于A、D组(P0.01),与B组差异不显著(P0.05)。D组Occludin表达水平明显高于A、B和C组,B组Occludin表达水平高于A组。综上,大鼠饮用水中添加香菇多糖可改善大鼠空肠形态结构,并提高其抵抗大肠杆菌感染能力,促进Occludin的表达。
[Abstract]:The purpose of this study was to investigate the effects of lentinan on the jejunum morphology, the number of epithelial cells and the expression of tight junction protein in the jejunum of rats infected with Escherichia coli.24 Sprague-Dawley rats were randomly divided into 4 groups with 6 repeats in each group, and 20 渭 g / mL lentinan was added to the distilled water in each group.On the 15th day of the experiment, the concentration of 2 mL was 1 脳 1010CFU/m L Escherichia coli K88 Agna C group, and the same amount of normal saline was given to BU D group.On the 18th day of the experiment, the heart was collected and executed, the jejunum was fixed and frozen, paraffin sections were made, hematoxylin-eosin (HEH) staining and Shevger's periodate (PASS) staining were used to determine the villi height, crypt depth and villi width.The ratio of villi height to crypt depth was calculated and the expression of jejunum tight junction protein (Occludin) was determined by Western-blot method.The results showed that there was no significant difference in villi height and villus width among three groups. The depth of crypt in group P0.05. C was significantly lower than that in group A B (P 0.01), and the number of IEL in group C was significantly lower than that in group D (P 0.05), and the number of IEL in group C was significantly lower than that in group A (P 0.01).The number of GC in group C was significantly higher than that in group A (P 0.01), but the expression of Occludin in group D was significantly higher than that in group A (P 0.05). The expression of Occludin in group C was significantly higher than that in group A (P 0.05).In conclusion, the addition of lentinan in drinking water of rats can improve the morphology and structure of jejunum, improve the ability of resisting Escherichia coli infection, and promote the expression of Occludin.
【作者单位】：安徽科技学院动物科学学院;
【基金】：国家自然科学基金项目(31501968) 安徽省高校自然科学研究项目(KJ2015A296) 安徽省自然科学基金(1608085QC72) 安徽科技学院大学生创新创业训练计划项目(16XCX01)
【分类号】：S816

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