柔嫩艾美耳球虫江苏株SAG10基因的克隆及分析

发布时间：2018-04-19 11:10

本文选题：柔嫩艾美耳球虫 + SAG基因　；参考：《江苏农业科学》2017年03期

【摘要】：根据Gen Bank中已发表的柔嫩艾美耳球虫(Eimeria tenella)SAG10基因序列,利用计算机软件设计1对引物,从E.tenella江苏株的第2代裂殖子中成功克隆出SAG10基因。将该基因与Gen Bank中国内外不同E.tenella分离株的SAG10基因进行比对,发现E.tenella江苏株与北京株的SAG10基因同源性最高,高达99%,其后依次为杨凌株、豪顿株。4种不同分离株SAG10基因的ORF之间有11个碱基不同,其中3个为无义突变,8个为有义突变。分析4种分离株SAG10基因推导蛋白质的亲水性、抗原指数、表面可及性发现,国内外不同E.tenella分离株SAG10蛋白的抗原性总体比较接近且很强,但国内不同分离株之间的抗原性更加接近,并优于国外的豪顿株,这可能是由于氨基酸突变引起蛋白质的空间构象和亲水性等发生变化而引起。将上述SAG10基因序列与复顶门原虫的其他相关SAGs基因序列进行遗传进化分析,发现Eimeria属球虫SAGs基因之间相对比较保守,且与犬新孢子虫(Neospora caninum)、N.hughesi、刚地弓形虫(Toxoplasma gondii)的SAG基因之间同源性较低,与已有观点一致;但神经肉孢子虫(Sarcocystis neurona)的2条SAG1基因序列却与E.tenella的SAG1基因序列表现出更高的同源性,这与其他研究结论不符。
[Abstract]:According to the published sequence of Eimeria tenella)SAG10 gene of Eimeria tenella in Gen Bank, a pair of primers were designed by computer software to clone SAG10 gene from the second generation merozoite of E.tenella Jiangsu strain.Comparing this gene with SAG10 gene of different E.tenella isolates of Gen Bank in China, we found that the homology of SAG10 gene between E.tenella Jiangsu strain and Beijing strain was the highest, up to 99%, followed by Yang Ling strain.There were 11 different bases in ORF of SAG10 gene in 4 strains of Haoton strain, of which 3 were nonsense mutations and 8 were semantic mutations.By analyzing the hydrophilicity, antigen index and surface accessibility of four SAG10 gene derived proteins, it was found that the antigenicity of SAG10 proteins of different E.tenella isolates at home and abroad was similar and strong.However, the antigenicity of different isolates in China is closer and better than that of overseas Haoton strains, which may be due to the changes in the spatial conformation and hydrophilicity of proteins caused by amino acid mutations.The genetic evolution analysis of the SAG10 gene sequence and other related SAGs gene sequences of protozoa was carried out. It was found that the SAGs gene of Eimeria belongs to coccidia was relatively conserved.The SAG gene of Toxoplasma gondii (Toxoplasma gondii) and Neospora caninatum (N. hughesii) had low homology, but the two SAG1 genes of Sarcocystis neuronashowed higher homology with E.tenella SAG1 gene.This is inconsistent with other studies.
【作者单位】：南通出入境检验检疫局;南通大学实验动物中心;江苏省疾病预防控制中心;
【基金】：国家自然科学基金(编号:31301926) 江苏省自然科学基金(编号:BK20130388)
【分类号】：S852.7

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