GDF9基因多态性对山羊血清FSH和LH水平的影响

发布时间：2018-04-21 01:40

本文选题：GDF基因 + 安徽白山羊　；参考：《畜牧与兽医》2017年12期

【摘要】：为了研究GDF9基因多态性与多胎山羊血清促卵泡素(follicle stimulating hormone,FSH)和促黄体素(lutein hormone,LH)水平的关联,试验选择经GDF9基因型鉴定后的自然发情健康经产安徽白山羊母羊80只,采集血样,应用PCR-SSCP检测GDF9基因的多态性,用全自动化学发光免疫分析法(CLIA)测定FSH和LH水平。结果表明:安徽白山羊群体中GDF9基因外显子存在2个SNPs,均出现3种基因型,在EF4461682:c.1956AC位点,不同基因型母羊外周血中FSH水平无明显差异,但CC型外周血中LH水平高于AA型(P005)。CC型和AC型产羔数(LSM)没有差异,但都显著高于AA型(P005);在EF4461682:c.3319GA位点,AA型外周血中FSH水平显著高于GG型(P005);AA型外周血中LH水平极显著高于GA和GG型(P001),GA型外周血中LH水平显著高于GG型(P005)。AA型平均产羔数LSM极显著高于AG型和GG型(P001),AG型平均产羔数LSM极显著高于GG型(P001)。由此得知,GDF9基因作为影响安徽白山羊产羔数的主效基因之一,其功能表达对FSH和LH水平起着重要影响。
[Abstract]:In order to study the association between GDF9 gene polymorphism and serum follicle stimulating hormone (FSH) and lutein hormonein (LHH) levels in multifetal goats, 80 healthy Anhui white goat estrous ewes with natural estrus, identified by GDF9 genotype, were selected and blood samples were collected. The polymorphism of GDF9 gene was detected by PCR-SSCP and the levels of FSH and LH were detected by chemiluminescence immunoassay (CLIA). The results showed that there were two SNPs in the exon of GDF9 gene and three genotypes in Anhui white goat population. There was no significant difference in FSH level in peripheral blood of different genotype ewe at EF4461682:c.1956AC locus. However, the level of LH in peripheral blood of CC type was higher than that of P005. CC type and AC type LSMs. The level of FSH in peripheral blood of EF4461682:c.3319GA locus was significantly higher than that of GG type P005A type, and the level of LH was significantly higher than that of GA and GG type P001 P001A type of peripheral blood LH level, which was significantly higher than that of LSM pole of GG type P005A type. The average lambs of AG type and GG type were significantly higher than those of AG type and GG type P001G type, and the average lambing number of AG type was significantly higher than that of GG type P001. It is concluded that GDF9 gene is one of the major genes affecting the litter size of Anhui white goats, and its functional expression plays an important role in the levels of FSH and LH.
【作者单位】：安徽省农业科学院畜牧兽医研究所;
【基金】：安徽省农业科学院院长青年创新基金项目(15b0415)
【分类号】：S827

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