猪7种腹泻相关病毒的临床检测及猪嵴病毒的遗传进化分析

发布时间：2018-04-21 08:15

本文选题：多重RT-PCR + 猪嵴病毒　；参考：《生物工程学报》2017年08期

【摘要】：为了解引起猪腹泻疫病的病毒性病原种类及感染现状,本研究首先针对7种报道较少但可引起猪腹泻的病毒性病原建立了多重RT-PCR检测方法,包括猪捷申病毒(PTV)、猪萨佩罗病毒(PSV)、猪丁型冠状病毒(PDCo V)、猪嵴病毒(PKV)、猪札幌病毒(PSa V)、猪星状病毒(PAst V)和猪环曲病毒(PTo V)。利用该方法对419份临床腹泻粪便样品进行筛检,结果显示PKV检出率最高,阳性率达26.98% 45.79%;PKV和其他病原混合感染率达9.52% 18.54%。基于PKV的高检出率及其可能在猪腹泻疾病中发挥的作用,本试验进一步选取3个PKV阳性样品进行全基因组序列测定及遗传进化分析。结果表明,这3个阳性样品PKV均归属于猪嵴病毒属,且与其他动物嵴病毒遗传距离较远,分别标记为CM-PKV、JS-PKV和PD-PKV;它们的全基因组同源性为88.1% 89.1%;CM-PKV与2013年报道的中国株JS-02a-CHN/2013同源性最高,而JS-PKV和PD-PKV则与2008年报道的匈牙利株K-30-HUN/2008/HUN同源性最高。表明上海不同地区猪群中存在的PKV有明显的遗传差异,但毒株遗传特性的差异与其致病力的相关性需进一步研究。本研究为深入了解PKV的流行现状及探讨其在猪腹泻疫情中的作用提供了参考。
[Abstract]:In order to understand the types of viral pathogens and the present situation of infection, a multiplex RT-PCR method was established for the detection of seven viral pathogens which are less reported but can cause pig diarrhea. These include PTV, PTV, PDCo, PKV, PSA, PST, PTO and PTO. The method was used to screen 419 fecal samples of clinical diarrhea. The results showed that the detection rate of PKV was the highest, the positive rate was 26.98% and the mixed infection rate of PKV and other pathogens was 9.52% and 18.54%. Based on the high detection rate of PKV and its possible role in porcine diarrhea, three PKV positive samples were selected for genome sequencing and genetic evolution analysis. The results showed that all of the three positive samples belonged to the genus porcine cristae, and had a long genetic distance from other animal cristae viruses, which were labeled CM-PKV JS-PKV and PD-PKV, respectively, and had the highest homology with Chinese strain JS-02a-CHN/2013 reported in 2013. JS-PKV and PD-PKV had the highest homology with the Hungarian strain K-30-HUN/2008/HUN reported in 2008. The results indicated that PKV in pig populations in different areas of Shanghai had obvious genetic differences, but the correlation between genetic characteristics of virulent strains and pathogenicity of the strains needed further study. This study provides a reference for further understanding of the prevalence of PKV and its role in swine diarrhea.
【作者单位】：上海市农业科学院畜牧兽医研究所上海种猪工程技术研究中心;上海海洋大学水产与生命学院;
【基金】：上海市科技兴农重点攻关项目(Nos.2013-5-5,2013-3-6) 上海市生猪产业体系项目(No.2016-6)资助~~
【分类号】：S852.651

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