半胱胺盐酸盐对泌乳期奶牛瘤胃发酵、生产性能及生化指标的安全性评价

发布时间：2018-04-21 12:32

  本文选题：半胱胺盐酸盐 + 瘤胃发酵　； 参考：《扬州大学》2015年硕士论文

【摘要】：本试验研究不同添加水平半胱胺盐酸盐(Cysteamine hydrochloride, CSH)对泌乳中期中国荷斯坦奶牛瘤胃发酵、生产性能和生化指标的影响。选用40头产奶量、泌乳日龄、胎次相近的奶牛,随机分为5组,每组8头,每组日粮中CSH添加量分别为：0g/头(对照组)、15g/头、30g/头、75g/头和150g/头,预试期14d,正试期56d,结果如下：1.CSH对泌乳奶牛瘤胃发酵特性、微生物蛋白合成及瘤胃细菌的影响,结果表明：各组奶牛的瘤胃液pH范围为6.30-6.69,组间差异不显著(P0.05),且各试验组乙酸含量与对照组相比无显著差异(P0.05)；75g组的氨态氮(NH3-N)浓度显著高于对照组和15g组(P=0.001),但75g和150g组总挥发性脂肪酸(VFA)浓度低于对照组和15g组(P=0.007),且其乙酸与丙酸比值显著低于15g组(P=0.004)。75g处理组原虫蛋白(P=0.018)及总菌体蛋白(P=0.041)的合成量最低,显著低于对照组。添加3Og/d-75g/d CSH提高了蛋白分解菌P. ruminicola(P=0.001)和B. fibrisolvens (P0.001)的相对数量,而150g/dCSH处理显著降低了F. succinogenes, R. albus和R. flavefaciens (P0.001)的相对数量。2.CSH对奶牛营养物质消化率、生产性能及乳成分的影响,结果表明：15g处理组的CP、Ca、P、NDF和GE表观消化率均高于对照组,但差异不显著(P0.05),而在75g-150g处理组呈一定降低趋势,150g处理组各营养指标消化率最低,其CP、NDF和ADF消化率显著低于对照组(P0.05)；奶牛的日均采食量、产奶量、4%标准乳产量及饲料转化率在CSH添加量为75g时最高,150g组最低,且150g处理组的产奶量和4%标准乳产量显著低于对照组及15g处理组(P0.05)；乳脂率和乳总固形物浓度在CSH添加量为75g时最高,且其他试验组与对照组无显著差异(P0.05),乳尿素氮浓度随着CSH添加量的升高而逐渐下降,且添加量为150g时最低(P0.05)。3.CSH对奶牛血清生化、酶、免疫、抗氧化能力及激素水平的影响,结果表明：当饲粮中CSH添加量为15 g时,奶牛血清中球蛋白水平显著高于150 g组(P0.05)；添加150gCSH组的谷丙转氨酶水平显著高于对照组及15和30gCSH处理组(P0.05)； CSH添加量30g组,T3水平显著高于对照组(P0.05)；添加量为15g时,血清T4水平显著高于对照组(P0.05)；15g-30g处理组的GH含量显著高于对照组(P0.05)；随着CSH添加量的增加,血清超氧化物歧化酶水平有下降趋势(0.05P0.1)；CSH添加量为15 g时,血清中谷胱甘肽过氧化物酶活性显著高于对照组和150g处理组(P0.05)；30g-150g处理组丙二醛水平高于对照组和15g处理组。15-30g/d处理组的IgA、IgG、IL-6、IL10和皮质醇浓度均高于对照组。其中,15-30g/d处理组的IgA浓度显著高于对照组和150g处理组(P0.05)；而15g处理组的IgG和IL-10水平显著高于75g-150g处理组(P0.05)；同时,15g处理组血清皮质醇浓度最高,显著高于150g处理组(P0.05)。结论：在本试验中,添加15-30g/dCSH能够促进奶牛瘤胃发酵、营养物质消化,降低血清酶的浓度,提高血清免疫、抗氧化指标水平,而添加75g-150gCSH时,对奶牛有一定副作用。因此,半胱胺盐酸盐在泌乳奶牛日粮中：安全剂量为15-75g/d,适宜剂量为15-30g/d,而从使用效果和经济角度考虑,最佳剂量为15g/d。
[Abstract]:The effects of Cysteamine hydrochloride (CSH) on the rumen fermentation, production performance and biochemical indexes of Chinese Holstein cows in the middle period of lactation were studied in this experiment. 40 cows were randomly divided into 5 groups, 8 heads in each group, and the amount of CSH added in each group was 0g/ head (0g/ head respectively). The control group), 15g/ head, 30g/ head, 75g/ head and 150g/ head, pre test period 14d and positive test period 56d, the results are as follows: 1.CSH has the effect of 1.CSH on ruminal fermentation characteristics, microbial protein synthesis and ruminal bacteria of lactating dairy cows. The results show that the pH range of the tumor gastric juice in each group is 6.30-6.69, and the difference between the groups is not significant (P0.05), and the acetic acid content and control of each test group are compared with the control group. The concentration of ammonia nitrogen (NH3-N) in group 75g was significantly higher than that of control group and 15g group (P=0.001), but the concentration of total volatile fatty acid (VFA) in 75g and 150g groups was lower than that of control group and 15g group (P=0.007), and the ratio of acetic acid to propionic acid was significantly lower than that of 15g group (P=0.004) and total bacterial protein (VFA) in group 75g and 150g. 41) was the lowest, significantly lower than the control group. Adding 3Og/d-75g/d CSH increased the relative number of P. ruminicola (P=0.001) and B. fibrisolvens (P0.001) of proteolytic bacteria, while 150g/dCSH treatment significantly reduced the succinogenes of F. and the relative quantity of R. and vegetables. The effects of performance and milk composition showed that the apparent digestibility of CP, Ca, P, NDF and GE in the 15g treatment group was higher than that of the control group, but the difference was not significant (P0.05), but in 75g-150g treatment group, the digestibility of the nutritional indexes in the 150g treatment group was the lowest, and the CP, NDF and ADF digestibility were significantly lower than that of the control group. Quantity, milk production, 4% standard milk yield and feed conversion rate were the highest when CSH was added to 75g, 150g group was the lowest, and the milk yield and 4% standard milk yield of 150g treatment group were significantly lower than that of the control group and 15g treatment group (P0.05); the milk fat rate and the total solid substance concentration were the highest when the CSH addition was 75g (P), and there was no significant difference between the other test groups and the control group (P). 0.05), the concentration of milk urea nitrogen gradually decreased with the increase of CSH addition, and the effect of the lowest (P0.05).3.CSH on serum biochemical, enzyme, immunity, antioxidant capacity and hormone levels of dairy cows was added to 150g. The results showed that the level of globulin in milk bovine serum was significantly higher than that of 150 g group (P0.05) when CSH was added to the diet of 15 g (P0.05). The level of alanine transaminase in 0gCSH group was significantly higher than that of control group and 15 and 30gCSH treatment group (P0.05); CSH added 30g group, T3 level was significantly higher than that of control group (P0.05); the level of serum T4 was significantly higher than that of control group (P0.05) when the amount of 15g was added, and the GH content of 15g-30g treatment group was higher than that of the control group; with the increase of addition, blood was increased. The level of superoxide dismutase was decreased (0.05P0.1); when the amount of CSH was 15 g, the activity of glutathione peroxidase in the serum was significantly higher than that of the control group and the 150g treatment group (P0.05). The level of malondialdehyde in the 30g-150g treatment group was higher than that of the control group and the 15g treatment group at IgA, IgG, IL-6, IL10 and cortisol concentration were higher than those in the control group. In the control group, the concentration of IgA in the 15-30g/d treatment group was significantly higher than that in the control group and the 150g treatment group (P0.05), while the IgG and IL-10 levels in the 15g treatment group were significantly higher than that in the 75g-150g treatment group (P0.05). At the same time, the serum cortisol concentration in the 15g treatment group was highest, significantly higher than that in the 150g treatment group (P0.05). In the cow rumen fermentation, digestion of nutrients, reducing the concentration of serum enzymes, improving the serum immunity and the level of antioxidant index, the cows have some side effects when adding 75g-150gCSH. Therefore, cysteamine hydrochloride is in the lactating dairy cow diet: the safe dose is 15-75g/d, and the suitable dose is 15-30g/d, which is considered from the use effect and the economic perspective. The best dose is 15g/d.

【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823

【相似文献】

相关期刊论文 前10条

1 杨磊;陈庆锋;谭国华;唐佰云;;半胱胺盐酸盐对泌乳功能的影响[J];饲料研究;2008年04期

2 林浩然,张皎;半胱胺盐酸盐对鳗鱼生长性能的影响[J];饲料广角;2005年11期

3 沈赞明,张荣飞,解红梅,陈峰,陆天水;半胱胺盐酸盐对泌乳20～42周奶牛产奶性能和部分免疫指标的影响[J];畜牧兽医学报;2005年07期

4 张荣飞;沈赞明;;半胱胺盐酸盐对高温季节奶牛生产性能的影响[J];动物营养学报;2007年02期

5 巩德球;陆逵;傅萃;金桩;李德志;;半胱胺盐酸盐对育肥猪生产性能的影响[J];饲料工业;2012年19期

6 沈赞明,申谊东,李嘉男,陈峰,陆天水;复合半胱胺盐酸盐对泌乳中期奶牛产奶性能的影响[J];中国畜牧兽医;2003年03期

7 陈丛亮;牛瑞萍;杨新社;张治涛;杨洪勋;杨磊;游丕荣;;不同营养水平下半胱胺盐酸盐对生长猪生产性能的影响[J];饲料研究;2009年11期

8 李余霞;谭青松;;半胱胺盐酸盐对水产养殖动物生理调节的研究[J];饲料研究;2009年04期

9 陈丛亮;杨洪勋;杨磊;武翠;顾晓峰;富伟林;;半胱胺盐酸盐对生长猪生产性能的影响[J];饲料研究;2009年09期

10 张红梅;;半胱胺盐酸盐对7日龄肉仔鸡免疫指标的影响[J];生物技术;2009年05期

相关会议论文 前10条

1 沈赞明;解红梅;;半胱胺盐酸盐减缓山羊手术后细胞免疫抑制效应的试验研究[A];动物生理生化学分会第八次学术会议暨全国反刍动物营养生理生化第三次学术研讨会论文摘要汇编[C];2004年

2 杜刚;张治涛;陈丛亮;刘雅石;;半胱胺盐酸盐β-环糊精微粒对母猪繁殖性能的影响研究[A];第六次全国饲料营养学术研讨会论文集[C];2010年

3 沈赞明;解红梅;;半胱胺盐酸盐减缓山羊手术后细胞免疫抑制效应的试验研究[A];动物生理生化学分会第八次学术会议暨全国反刍动物营养生理生化第三次学术研讨会论文摘要汇编[C];2004年

4 张磊;沈赞明;;半胱胺盐酸盐对手术山羊外周血白细胞数量及淋巴细胞转化率的影响[A];全国动物生理生化第十次学术交流会论文摘要汇编[C];2008年

5 沈赞明;何军;曹利;;半胱胺盐酸盐促进手术损伤山羊小肠粘膜的再生和原代培养奶牛十二指肠细胞增殖的研究[A];中国生理学会2007年消化内分泌生殖学术研讨会论文摘要汇编[C];2007年

6 沈赞明;何军;;半胱胺盐酸盐对断奶羔山羊小肠发育的影响[A];中国生理学会2007年消化内分泌生殖学术研讨会论文摘要汇编[C];2007年

7 杜刚;张治涛;陈丛亮;刘雅石;;半胱胺盐酸盐β-环糊精微粒对母猪繁殖性能的影响研究[A];《2010中国猪业进展》论文集[C];2010年

8 沈赞明;张荣飞;;半胱胺盐酸盐对热应激状态下高产奶牛泌乳后期生产性能的影响[A];动物生理生化学分会第八次学术会议暨全国反刍动物营养生理生化第三次学术研讨会论文摘要汇编[C];2004年

9 张荣飞;沈赞明;;半胱胺盐酸盐对高温季节高产奶牛泌乳中期生产性能的影响[A];动物生理生化学分会第八次学术会议暨全国反刍动物营养生理生化第三次学术研讨会论文摘要汇编[C];2004年

10 何军;沈赞明;;半胱胺盐酸盐缓解山羊胃肠道手术应激及其抗损伤机制研究[A];全国动物生理生化第九次学术交流会论文摘要汇编[C];2006年

相关硕士学位论文 前8条

1 刘威;半胱胺盐酸盐饲喂泌乳中期奶牛的效果评价[D];甘肃农业大学;2014年

2 张荣飞;半胱胺盐酸盐对高温季节高产奶牛生产性能的影响[D];南京农业大学;2004年

3 解红梅;半胱胺盐酸盐和两种必需氨基酸对山羊细胞免疫功能的影响[D];南京农业大学;2004年

4 邓惠中;半胱胺盐酸盐对AA肉鸡生产性能、血清生化指标及肠道菌群的影响[D];湖南农业大学;2011年

5 肖凤;半胱胺盐酸盐的合成工艺研究[D];重庆大学;2007年

6 刘红;半胱胺盐酸盐对泌乳期奶牛瘤胃发酵、生产性能及生化指标的安全性评价[D];扬州大学;2015年

7 曹利;半胱胺对手术山羊外周血T淋巴细胞作用的研究及其抗氧化作用初探[D];南京农业大学;2008年

8 范俊明;不同日粮条件下半胱胺盐酸盐和两种必需氨基酸对山羊瘤胃消化代谢影响的研究[D];南京农业大学;2004年

，

本文编号：1782447