中国地方猪种线粒体基因组遗传多样性及其系统发育分析

发布时间：2018-04-22 00:18

本文选题：中国地方猪 + 线粒体DNA　；参考：《中国畜牧兽医》2017年08期

【摘要】：为从分子水平上探究中国地方猪种遗传多样性和分类地位,本试验采用生物信息学方法比较了6个类型共22个中国地方猪种的线粒体基因组全序列,分析了其多态性,并构建了6个类型猪种线粒体D-loop区单倍型的网络中介图以及基于线粒体D-loop序列、Cytb基因、完整编码区序列的系统进化树。结果表明,6个类型22个猪种中共检测到了144个多态位点,22种单倍型,说明地方猪种具有丰富的遗传多样性;地方猪线粒体基因组序列中核苷酸变异以转换为主,且Ti/Tv大于转换/颠换比临界值(2.0),变异位点均符合中性突变。6个类型猪种间遗传距离均较小,且有共享单倍型。系统进化树结果表明,6种类型地方猪种主要聚为两个支系。表明线粒体D-loop序列及Cytb基因均可作为研究种内系统发育、起源进化的分子标记。
[Abstract]:In order to explore the genetic diversity and taxonomic status of Chinese local pig breeds at the molecular level, the total mitochondrial genome sequences of 6 types of 22 local Chinese pigs were compared and their polymorphisms were analyzed by bioinformatics. The network intermediary map of mitochondrial D-loop haplotype and phylogenetic tree based on mitochondrial D-loop gene and complete coding region were constructed in 6 pig breeds. The results showed that a total of 144 polymorphic loci and 22 haplotypes were detected in 22 pig breeds of 6 types, indicating that local pig breeds have abundant genetic diversity, and that the nucleotide variation in mitochondrial genome sequence of local pigs is dominated by transformation. The Ti/Tv was larger than the critical value of the conversion / transversion ratio, and the mutation sites were all in accordance with neutral mutation. The genetic distances among the six types of pigs were smaller and shared haplotypes. Phylogenetic tree results showed that six local pig breeds were mainly clustered into two branches. The results showed that mitochondrial D-loop sequence and Cytb gene could be used as molecular markers to study the phylogeny and evolution of intraspecific phylogeny.
【作者单位】：河北农业大学动物科技学院;国家北方山区农业工程技术研究中心;河北省山区农业工程技术研究中心;河北工程大学生命科学与食品工程学院;
【基金】：河北省现代农业产业技术体系专项(HBCT2013070202)
【分类号】：S828

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