苜草素对产蛋后期蛋鸡抗氧化能力、脂质代谢及相关基因表达的影响

发布时间：2018-04-22 08:46

本文选题：苜草素 + 产蛋后期蛋鸡　；参考：《动物营养学报》2017年04期

【摘要】：本试验旨在研究不同水平苜草素对产蛋后期蛋鸡抗氧化能力、脂质代谢及相关基因表达的影响。选用69周龄、健康海兰褐蛋鸡324只,随机分为3组,每组6个重复,每个重复18只鸡。对照组饲喂基础饲粮,试验组在基础饲粮的基础上添加500和1 000 mg/kg苜草素。试验预试期2周,正试期8周。结果表明,与对照组相比:1)试验组蛋鸡产蛋率显著升高(P0.05),料蛋比显著降低(P0.05)。2)500 mg/kg苜草素显著增加了蛋壳强度(P0.05),极显著降低了肝脏甘油三酯含量(P0.01),显著降低了肝脏总胆固醇含量(P0.05)。3)1 000mg/kg苜草素极显著提高了血浆谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)及肝脏GSH-Px活性(P0.01),显著提高了血浆总抗氧化能力(T-AOC)、降低了丙二醛(MDA)的含量(P0.05),极显著降低了肝脏与蛋黄总胆固醇和甘油三酯含量(P0.01),极显著提高了肝脏硫氧还蛋白还原酶1(Trx R1)、胆固醇-7α羟化酶(CYP7A1)、固醇调节元件结合蛋白-1c(SREBP-1c)mRNA表达量(P0.01),极显著降低了3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA表达量(P0.01),而对GSH-Px mRNA表达量无显著影响(P0.05)。由此可见,饲粮中添加苜草素可降低产蛋后期蛋鸡蛋黄胆固醇和甘油三酯含量,提高生产性能、蛋壳强度、机体抗氧化和胆固醇代谢能力,且以添加1 000 mg/kg苜草素效果较好。
[Abstract]:The purpose of this study was to study the effects of different levels of clovenin on antioxidant capacity, lipid metabolism and related gene expression in laying hens at late laying stage. A total of 324 healthy Hailan brown laying hens aged 69 weeks were randomly divided into 3 groups with 6 replicates in each group and 18 chickens in each replica. The control group was fed with basic diet, and the experimental group was supplemented with 500 and 1 000 mg/kg clover on the basis of basic diet. The pre-test period was 2 weeks and the positive test period was 8 weeks. The results show that Compared with the control group, the laying rate of laying hens in the test group was significantly higher than that of the control group, and the feed / egg ratio was significantly lower than that of the control group. The ratio of feed to egg decreased significantly, P0.05N increased the eggshell strength and P0.05N significantly decreased the content of triglyceride in the liver and the content of total cholesterol in the liver. The plasma glutathione peroxidase (GSH-PxX), total superoxide dismutase (T-SOD) and liver GSH-Px activity (P0.01C) were significantly increased, the plasma total antioxidant capacity (T- AOCN) and the content of malondialdehyde (MDA) were decreased significantly. The contents of total cholesterol and triglyceride in liver and egg yolk were significantly increased by 1(Trx R1, CYP7A1, steroid regulatory element binding protein (SREBP-1cN) and 3hydroxy-3-methyl-methyl-1, respectively. The mRNA expression of glutaryl coenzyme A reductase (HMGCRN) was P0.01, but the expression of GSH-Px mRNA was not significantly affected. It can be seen that the addition of clovenin in diet can reduce the content of cholesterol and triglyceride in egg yolk, improve the production performance, eggshell strength, body antioxidant and cholesterol metabolism ability, and the addition of 1 000 mg/kg clover is better.
【作者单位】：山西农业大学动物科技学院;
【基金】：山西省科学技术发展计划项目(201603D221033-1) 山西省现代农业鸡产业技术体系(20161102) 山西农业大学博士科研启动基金(412559)
【分类号】：S831.5

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