红曲霉素中药合生元对京红鸡生长性能及免疫功能的影响

发布时间：2018-04-22 22:24

本文选题：红曲霉素 + 中草药合生元　；参考：《延边大学》2015年硕士论文

【摘要】：本课题的目的是研究红曲霉素中药合生元对京红鸡的生长性能、血液常规指标以及免疫器官指数的影响。从而达到开发无污染、无残留、安全可靠的绿色饲料添加剂来代替抗生素,提高畜禽的免疫力,降低其饲料成本,提高畜禽产品的质量的目的。本实验选用90只1日龄的京红鸡投入试验。随机分为3组,2个试验组(高剂量组1%和低剂量组0.5%,)和1个空白组。分别在雏鸡饲料中添加红曲霉素中药合生元添加剂0.5%(组1)、1%(组2),将添加剂混在基础饲料中连续给药30d。空白组饲喂基础饲料(其中不添加任何添加剂)。在试验期内测定了京红鸡的生产性能指标,30d喂养试验后测定了京红鸡的血液常规指标,免疫器官指数的指标以及对免疫器官进行组织切片观察对比的试验。试验结果如下：1、在测定的京红鸡生长性能指标当中,针对京红鸡的采食量和日增重量以及饲料的利用率测定结果显示,京红鸡在受试30 d后,试验1组和试验2组与空白组之间增重、采食量及饲料利用率差异稍显著(P0.05)。其中,试验1组较空白组的饲料利用率提高了17.02%,试验2组较空白组的饲料利用率提高了16.29%。但试验1组与试验2组之间差异不显著(P0.05)。2、在测定的京红鸡血液常规指标当中,京红鸡在受试30d后,试验1组和试验2组与空白组之间的各项指标差异稍显著(P0.05)。其中,试验1组比空白组淋巴细胞的含量比提高了17.63%,试验2组比空白组淋巴细胞的含量比提高了16.72%。但试验1组与试验2组之间差异不显著(P0.05)。3、通过对空白组以及试验1、2组的鸡进行剖检观察得到试验1组与试验2组较空白组的脾脏有明显的增大变化。同时试验2组在肝脏称重时可见大部分肝脏有肿大现象。4、在测定的京红鸡免疫器官的脏器系数指标当中,在给予京红鸡受试物30d后,试验1组和试验2组之间脏器系数差异不显著(P0.05),但两者与空白组之间差异稍显著(P0.05)。5、通过对京红鸡免疫器官的组织切片对比试验观察中得出,分别选用高低倍镜在微细结构中观察发现脾脏、肝脏没有明显不同变化,但胸腺当中发现试验1组和试验2组小淋巴细胞的密集程度较空白组之间较多。法氏囊中发现实验1组与试验2组的法氏囊皱襞相比空白组的要明显的增大、增厚,并且在法氏囊的髓质部分出现染色较淡的未分化的上皮样细胞和空泡样物,其中试验1组空泡样物数量不明显,试验2组稍明显,正常组十分明显。目前,国内外单独性地将红曲霉素或中药合生元作为饲料添加剂的研究较多,但将二者合用添加的研究甚少。因此本试验开展了红曲霉素中药合生元对京红鸡生理、生化指标及免疫器官影响的研究,以期促进畜牧业以及饲料业的绿色健康发展。
[Abstract]:The purpose of this study was to study the effects of erythromycin on growth performance, blood routine index and immune organ index of Jinghong chicken. So as to develop a green feed additive without pollution, residue, safety and reliability to replace antibiotics, improve the immunity of livestock and poultry, reduce its feed cost, and improve the quality of livestock and poultry products. A total of 90 1-day-old Jinghong chickens were used in the experiment. They were randomly divided into 3 groups, 2 experimental groups (1% in high dose group and 0. 5% in low dose group) and 1 blank group. The feed of chicks was fed with erythromycin combined with traditional Chinese medicine additive 0.5 (group 1) (group 1) (group 2), and the additive was mixed in the basic feed for 30 days. The blank group was fed with basic feed (no additive was added). The production performance index of Jinghong chicken was measured during the trial period. After 30 days of feeding test, the routine blood index, immune organ index and tissue section of immune organs were measured and compared. The results were as follows: 1. Among the growth performance indexes of Jinghong chicken, the results of feed intake, daily gain and feed utilization ratio showed that Jinghong chicken was tested 30 days later. There were significant differences in weight gain, feed intake and feed utilization efficiency between test group 1 and group 2 and blank group (P 0.05). Among them, the feed utilization rate of group 1 was 17.02 higher than that of blank group, and that of group 2 was 16.29% higher than that of blank group. However, there was no significant difference between group 1 and group 2 (P 0.05). Among the blood routine indexes of Jinghong chicken, the difference between test group 1 and group 2 and blank group was slightly significant (P 0.05) after 30 days of test. The lymphocyte content ratio in group 1 was 17.63 higher than that in control group, and that in group 2 was 16.72% higher than that in blank group. However, there was no significant difference between group 1 and group 2 (P 0.05). The spleen of group 1 and group 2 were obviously enlarged by the observation of the chickens in blank group and test group 1 and 2, compared with the blank group, the spleen of group 1 and group 2 were significantly larger than that of the control group (P < 0.05), but the difference between group 1 and group 2 was not significant (P 0.05). At the same time, most of the liver in group 2 showed swelling at the time of liver weighing. Among the visceral coefficients of the immune organs of Jinghong chicken, 30 days after the treatment of Jinghong chicken, There was no significant difference in visceral coefficient between group 1 and group 2, but there was a little significant difference between the two groups and the blank group. The spleen and liver were observed with high and low power microscope respectively, but the density of small lymphocytes in group 1 and group 2 was more than that in control group. It was found in the bursa of Fabricius that the folds of the bursa of the first group were significantly larger and thicker than those of the control group, and the undifferentiated epithelioid cells and vacuoles were found in the medulla of the bursa of Fabricius. The number of vacuoles in group 1 was not obvious, that in group 2 was slightly obvious, and that in normal group was very obvious. At present, there are many researches on Monascus mycin or Chinese medicine synbiotics as feed additives at home and abroad, but few studies have been done on the combination of them. In order to promote the green and healthy development of animal husbandry and feed industry, the study was carried out on the physiological, biochemical indexes and immune organs of Jinghong chicken.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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